Overview

  • Product nameAnti-Perilipin A+B antibody
    See all Perilipin A+B primary antibodies
  • Description
    Rabbit polyclonal to Perilipin A+B
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: MEPVVRRLST QFTAANELAC RGLDHLEEKI PALQYPPEKI ASELKDTIST, corresponding to a region within N terminal amino acids 73-122 of Human Perilipin A+B

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab66514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay:1:312500.

Target

  • RelevancePerilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body. Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.
  • Cellular localizationLipid droplet
  • Database links
  • Alternative names
    • Lipid droplet associated protein antibody
    • PERIA antibody
    • PERIB antibody
    • Perilipin A antibody
    • Perilipin antibody
    • Perilipin B antibody
    • PLIN antibody
    see all

Anti-Perilipin A+B antibody images

  • Anti-Perilipin A+B antibody (ab66514) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)

References for Anti-Perilipin A+B antibody (ab66514)

ab66514 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"