Overview

  • Product nameAnti-Periostin antibody
    See all Periostin primary antibodies
  • Description
    Rabbit polyclonal to Periostin
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Recombinant fusion protein (Human) produced in E. coli. (75 kDa, 648 amino acid residues of the protein 22-669 and 23 additional amino acid residues).

Properties

Applications

Our Abpromise guarantee covers the use of ab14041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 19079190
IHC-P 1/100 - 1/1000.
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 100 kDa.
ELISA 1/100 - 1/1000.

Target

  • FunctionBinds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.
  • Tissue specificityWidely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.
  • Sequence similaritiesContains 1 EMI domain.
    Contains 4 FAS1 domains.
  • Post-translational
    modifications
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fasciclin-I like antibody
    • MGC119510 antibody
    • MGC119511 antibody
    • OSF 2 antibody
    • OSF-2 antibody
    • OSF2 antibody
    • Osteoblast specific factor 2 (fasciclin I like) antibody
    • Osteoblast specific factor 2 antibody
    • Osteoblast specific factor antibody
    • Osteoblast-specific factor 2 antibody
    • PDLPOSTN antibody
    • Periodontal ligament specific periostin antibody
    • Periostin antibody
    • Periostin isoform thy2 antibody
    • Periostin isoform thy4 antibody
    • Periostin isoform thy6 antibody
    • Periostin isoform thy8 antibody
    • Periostin osteoblast specific factor antibody
    • PN antibody
    • POSTN antibody
    • POSTN_HUMAN antibody
    • RP11 412K4.1 antibody
    see all

Anti-Periostin antibody images

  • ab14041 at a 1/100 dilution staining Periostin in mouse dermis tissue by Immunohistochemistry (frozen sections) incubated for 16 hours at 4°C. PFA fixed. Permeabilized using 0.1% Triton. Blocked using 20% serum for 1 hour at 4°C. Secondary used at 1/200 polyclonal Donkey anti-rabbit conjugated to Alexa Fluor 555 (red). Counterstain (blue) DAPI.

    See Abreview

  • ab14041, staining Periostin in Human breast carcinoma tissue by Immunohistochemistry (Frozen sections).

    Sections were acetone-fixed and blocked in normal serum. The sections were incubated with the primary antibody (for 1 hour at room temperature), followed by incubation with an HRP-conjugated secondary antibody for additional 30 minutes at room temperature. Diaminobenzidine (DAB) was used as a chromogen resulting in brown staining of positive cells. The nuclei were counterstained in using hematoxylin.
  • ICC/IF image of ab14041 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14041, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Periostin antibody (ab14041)

This product has been referenced in:
  • Zhang Z  et al. Upregulated periostin promotes angiogenesis in keloids through activation of the ERK 1/2 and focal adhesion kinase pathways, as well as the upregulated expression of VEGF and angiopoietin-1. Mol Med Rep 11:857-64 (2015). IHC-P ; Human . Read more (PubMed: 25369801) »
  • Okada H  et al. Phenotype and physiological significance of the endocardial smooth muscle cells in human failing hearts. Circ Heart Fail 8:149-55 (2015). IHC-P ; Human . Read more (PubMed: 25466765) »

See all 40 Publications for this product

Product Wall

The selling size of ab14041 is 50ugand in lyophilized form. We recommend to reconstitute the product in 0.05ml deionized water. This would result in 1ug/ul (50ug in 0.05ml water).

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Jaculus jaculus Tissue sections (Metatarsals)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid based
Permeabilization No
Specification Metatarsals
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
Fixative Paraformaldehyde
Username

HAYDEE GUTIERREZ

Verified customer

Submitted Jun 08 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Periosteum Sheep Tibia)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinas K - Ready to use (Dako - S3020)
Permeabilization No
Specification Periosteum Sheep Tibia
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 11 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization No
Specification HeLa
Fixative Methanol
Username

Dr. Kirk McManus

Verified customer

Submitted Aug 07 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CC1
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 27 2015

Application Immunohistochemistry (Frozen sections)
Blocking step Blockace as blocking agent for 10 minute(s) · Concentration: 4% · Temperature: 4°C
Sample Dog Tissue sections (Skin)
Specification Skin
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 19 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step None
Sample Dog Tissue sections (Skin)
Specification Skin
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 19 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrition buffer
Sample Mouse Tissue sections (mouse embryo)
Specification mouse embryo
Permeabilization Yes - 1% triton x-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 02 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Sample Mouse Tissue sections (mouse embryo)
Specification mouse embryo
Permeabilization Yes - 1% triton x-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 02 2014

The concentration of ab14041 is reconstitution dependant e.g. if you dissolve the antibody in 50ul of ultra pure water the concentration will be 1mg/ml.

The concentration of ab117951 is lot dependant. It should be written on the vial. Please l...

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1-10 of 26 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"