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Recombinant fusion protein (Human) produced in E. coli. (75 kDa, 648 amino acid residues of the protein 22-669 and 23 additional amino acid residues).
Lots # GR310924-1, GR296166-4, and GR296166-5 are sold in lyophilised form. To reconstitute, add 100 µl of deionized water and let the lyophilised antibody dissolve completely. The resulting concentration is 0.5 mg/ml. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Our Abpromise guarantee covers the use of ab14041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 19079190|
|IHC-P||1/100 - 1/1000.|
|WB||Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 100 kDa.|
|ELISA||1/100 - 1/1000.|
ICC/IF image of ab14041 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14041, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab14041 staining Periostin in Mouse PDL tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, permeablized with Triton-X, blocked with 5% serum for 60 minutes at 25°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/200) at 4°C for 16 hours. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody. HRP-streptavidin and DAB for detection
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"