Overview

  • Product nameAnti-Periostin antibody
    See all Periostin primary antibodies
  • Description
    Rabbit polyclonal to Periostin
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
    Predicted to work with: Bird, Fish, all Mammals, Amphibians
  • Immunogen

    Synthetic peptide from the fasciclin domain 1 of mouse periostin.

  • Positive control
    • Rat lung lysate and mouse lung extract

Properties

Applications

Our Abpromise guarantee covers the use of ab92460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 93 kDa.
IHC-P 1/200.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionBinds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.
  • Tissue specificityWidely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.
  • Sequence similaritiesContains 1 EMI domain.
    Contains 4 FAS1 domains.
  • Post-translational
    modifications
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fasciclin-I like antibody
    • MGC119510 antibody
    • MGC119511 antibody
    • OSF 2 antibody
    • OSF-2 antibody
    • OSF2 antibody
    • Osteoblast specific factor 2 (fasciclin I like) antibody
    • Osteoblast specific factor 2 antibody
    • Osteoblast specific factor antibody
    • Osteoblast-specific factor 2 antibody
    • PDLPOSTN antibody
    • Periodontal ligament specific periostin antibody
    • Periostin antibody
    • Periostin isoform thy2 antibody
    • Periostin isoform thy4 antibody
    • Periostin isoform thy6 antibody
    • Periostin isoform thy8 antibody
    • Periostin osteoblast specific factor antibody
    • PN antibody
    • POSTN antibody
    • POSTN_HUMAN antibody
    • RP11 412K4.1 antibody
    see all

Anti-Periostin antibody images

  • Anti-Periostin antibody (ab92460) at 1/1000 dilution + rat lung lysate

    Predicted band size : 93 kDa
  • ICC/IF image of ab92460 stained HepG2 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92460, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab92460 staining in Hu Aorta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92460, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Periostin antibody (ab92460)

This product has been referenced in:
  • Jia W  et al. Coexpression of periostin and EGFR in patients with esophageal squamous cell carcinoma and their prognostic significance. Onco Targets Ther 9:5133-42 (2016). WB, IHC-P . Read more (PubMed: 27574454) »
  • Kim SS  et al. Nifedipine Induces Periostin Expression in Gingival Fibroblasts through TGF-beta. J Dent Res 92:1022-8 (2013). Read more (PubMed: 24004653) »

See all 3 Publications for this product

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DISCOUNT CODE: XXXXXXXXX
Expiration date: 08-01-2013
Value: £227

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"