Anti-Peripherin antibody [8G2] (ab4653)
Key features and details
- Mouse monoclonal [8G2] to Peripherin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-Peripherin antibody [8G2]
See all Peripherin primary antibodies -
Description
Mouse monoclonal [8G2] to Peripherin -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Recombinant Rat Peripherin purified from E. coli.
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Positive control
- WB: Mouse, rat, cow spinal cord lysate, SH-SY5Y, PC-12 whole cell lysate. ICC: SH-SY5Y, PC-12 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.032% Sodium azide
Constituents: 49.98% Glycerol, 49.98% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified antibody from TCS. -
Clonality
Monoclonal -
Clone number
8G2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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ICC/IF |
1/500.
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Notes |
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WB
1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
ICC/IF
1/500. |
Target
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Function
Class-III neuronal intermediate filament protein. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 510082 Cow
- Entrez Gene: 5630 Human
- Entrez Gene: 19132 Mouse
- Entrez Gene: 24688 Rat
- Omim: 170710 Human
- SwissProt: P41219 Human
- SwissProt: P15331 Mouse
- SwissProt: P21807 Rat
see all -
Alternative names
- NEF 4 antibody
- NEF4 antibody
- Neurofilament 4 (57kD) antibody
see all
Images
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All lanes : Anti-Peripherin antibody [8G2] (ab4653) at 1/500 dilution
Lane 2 : Mouse spinal cord lysate
Lane 3 : Rat spinal cord lysate
Lane 4 : Cow spinal cord lysate
Lane 5 : SH-SY5Y (human bone marrow neuroblastoma cell) whole cell lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
Secondary
All lanes : Anti-Mouse IgG
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 25 kDa (possible IgG), 50 kDa (possible IgG)The band at ~57kDa corresponds to the peripherin protein. Bands at 50 and 25kDa detected in the mouse spinal cord lysate, correspond to the heavy and light chains of mouse IgG.
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Immunofluorescent analysis of SH-SY5Y (human bone marrow neuroblastoma cell) cells (A) and PC-12 (rat adrenal gland pheochromocytoma cell) cells (B), stained with ab4653 at 1/500 dilution (Red) and costained with chicken polyclonal anti-vimentin antibody at 1/10,000 dilution (Green). Blue: DAPI staining of nuclear DNA.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab4653 has been referenced in 3 publications.
- Li L et al. Up-regulation of Cavß3 subunit in primary sensory neurons increases voltage-activated Ca2+ channel activity and nociceptive input in neuropathic pain. J Biol Chem 287:6002-13 (2012). IHC-FrFl ; Rat . PubMed: 22187436
- Brandao KE et al. A-kinase anchoring protein 150 expression in a specific subset of TRPV1- and CaV 1.2-positive nociceptive rat dorsal root ganglion neurons. J Comp Neurol 520:81-99 (2012). WB, ICC/IF ; Rat . PubMed: 21674494
- Errante L et al. The intermediate filament protein peripherin is a marker for cerebellar climbing fibres. J Neurocytol 27:69-84 (1998). PubMed: 9609398