Anti-Peripherin antibody (ab4666)
Key features and details
- Rabbit polyclonal to Peripherin
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Peripherin antibody
See all Peripherin primary antibodies -
Description
Rabbit polyclonal to Peripherin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length rat peripherin purified from E. coli.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (3) |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/2000. Detects a band of approximately 57 kDa.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/1000 - 1/2000. Detects a band of approximately 57 kDa. |
Target
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Function
Class-III neuronal intermediate filament protein. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 5630 Human
- Entrez Gene: 24688 Rat
- Omim: 170710 Human
- SwissProt: P41219 Human
- SwissProt: P21807 Rat
- Unigene: 37044 Human
- Unigene: 11086 Rat
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Alternative names
- NEF 4 antibody
- NEF4 antibody
- Neurofilament 4 (57kD) antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of mixed neuron/glia cultures from newborn rat brain labelling Peripherin (green) with ab4666 and Neurofilament heavy polypeptide (red) with ab4680. A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain Neurofilament heavy polypeptide and not peripherin. The blue channel reveals the localization of DNA.
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Anti-Peripherin antibody (ab4666) at 1/1000 dilution + Rat spinal cord homogenate
Observed band size: 57 kDa why is the actual band size different from the predicted? -
Anti-Peripherin antibody (ab4666) at 1 µg/ml +
Recombinant Human Peripherin protein (ab114335) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20 secondsSecondary antibody - goat anti-rabbit HRP preasorbed (ab97080)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (37)
ab4666 has been referenced in 37 publications.
- Cantor EL et al. Passage number affects differentiation of sensory neurons from human induced pluripotent stem cells. Sci Rep 12:15869 (2022). PubMed: 36151116
- Plumbly W et al. Derivation of nociceptive sensory neurons from hiPSCs with early patterning and temporally controlled NEUROG2 overexpression. Cell Rep Methods 2:100341 (2022). PubMed: 36452863
- Tian DH et al. Phenotypic and functional comparison of rat enteric neural crest-derived cells during fetal and early-postnatal stages. Neural Regen Res 16:2310-2315 (2021). PubMed: 33818517
- Zeidler M et al. NOCICEPTRA: Gene and microRNA Signatures and Their Trajectories Characterizing Human iPSC-Derived Nociceptor Maturation. Adv Sci (Weinh) 8:e2102354 (2021). PubMed: 34486248
- Dempsey B et al. A medullary centre for lapping in mice. Nat Commun 12:6307 (2021). PubMed: 34728601