Overview

  • Product name
  • Description
    Rabbit polyclonal to Peripherin
  • Host species
    Rabbit
  • Specificity
    ab53051 detects endogenous levels of total Peripherin protein.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthetic peptide derived from human Peripherin.

  • Positive control
    • Human brain tissue and HepG2 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab53051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

Images

  • All lanes : Anti-Peripherin antibody (ab53051) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HepG2 cell extract with immunising peptide

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • ab53051 at 1/50 dilution staining Peripherin in human brain by Immunohistochemistry, Paraffin embedded tissue (in the absence and presence of the immunising peptide).

References

ab53051 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung adenocarcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 8.5 buffer
Permeabilization
No
Specification
Lung adenocarcinoma
Fixative
Formaldehyde
Username

Mr. Allan Wang

Verified customer

Submitted Mar 20 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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