Anti-Periplakin antibody (ab72422)
Key features and details
- Rabbit polyclonal to Periplakin
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Periplakin antibody
See all Periplakin primary antibodies -
Description
Rabbit polyclonal to Periplakin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Guinea pig, Cow, Xenopus laevis, Chimpanzee, Amphibian, Rhesus monkey, Gorilla, Orangutan, Medaka fish -
Immunogen
Synthetic peptide corresponding to Human Periplakin.
Database link: O60437 -
Positive control
- WB: HeLa, NIH 3T3, TCMK-1, 4T1, CT26.WT, and C6 cells. IP: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab72422 was affinity purified using an epitope specific to Periplakin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 205 kDa (predicted molecular weight: 205 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 205 kDa (predicted molecular weight: 205 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling. -
Tissue specificity
Expressed in stratified squamous epithelia and in some other epithelia. -
Sequence similarities
Belongs to the plakin or cytolinker family.
Contains 2 plectin repeats.
Contains 4 spectrin repeats. -
Cellular localization
Cell junction > desmosome. Cytoplasm > cytoskeleton. Cell membrane. Nucleus. Mitochondrion. Associated with desmosomes and intermediate filaments. - Information by UniProt
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Database links
- Entrez Gene: 5493 Human
- Entrez Gene: 19041 Mouse
- Entrez Gene: 302934 Rat
- Omim: 602871 Human
- SwissProt: O60437 Human
- SwissProt: Q9R269 Mouse
- Unigene: 192233 Human
- Unigene: 266875 Mouse
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Alternative names
- 190 kDa paraneoplastic pemphigus antigen antibody
- 195 kDa cornified envelope precursor antibody
- 195 kDa cornified envelope precursor protein antibody
see all
Images
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All lanes : Anti-Periplakin antibody (ab72422) at 1/2000 dilution
Lane 1 : NIH 3T3 Whole cell lysate
Lane 2 : TCMK-1 Whole cell lysate
Lane 3 : 4T1 Whole cell lysate
Lane 4 : CT26.WT Whole cell lysate
Lane 5 : C6 Whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 205 kDa -
All lanes : Anti-Periplakin antibody (ab72422) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 205 kDa
Observed band size: 205 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds -
Detection of Periplakin by Western Blot of Immunprecipitate.
ab72422 at 1µg/ml staining Periplakin in HeLa whole cell lysate immunoprecipitated using ab72422 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab72422 has been referenced in 2 publications.
- Cottle DL et al. Topical Aminosalicylic Acid Improves Keratinocyte Differentiation in an Inducible Mouse Model of Harlequin Ichthyosis. Cell Rep Med 1:100129 (2020). PubMed: 33294854
- Goldie SJ et al. Loss of GRHL3 leads to TARC/CCL17-mediated keratinocyte proliferation in the epidermis. Cell Death Dis 9:1072 (2018). PubMed: 30341279