Overview

  • Product nameAnti-PERK antibody
    See all PERK primary antibodies
  • Description
    Rabbit polyclonal to PERK
  • SpecificityThis antibody detects endogenous levels of total PERK protein.
  • Tested applicationsSuitable for: ELISA, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthesized peptide derived from an internal region of human PERK

  • Positive control
    • HepG2 cells and extracts from Hela and HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab65142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 125 kDa).
IP Use at an assay dependent concentration.

Target

  • FunctionPhosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.
  • Tissue specificityUbiquitous. A high level expression is seen in secretory tissues.
  • Involvement in diseaseDefects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 1 protein kinase domain.
  • DomainThe lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP.
  • Post-translational
    modifications
    Autophosphorylated.
    N-glycosylated.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781H1925 antibody
    • E2AK3_HUMAN antibody
    • EC 2.7.11.1 antibody
    • Eif2ak3 antibody
    • Eukaryotic translation initiation factor 2 alpha kinase 3 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 3 antibody
    • Heme regulated EIF2 alpha kinase antibody
    • HRI antibody
    • HsPEK antibody
    • Pancreatic eIF2 alpha kinase antibody
    • Pancreatic eIF2-alpha kinase antibody
    • PEK antibody
    • PRKR like endoplasmic reticulum kinase antibody
    • PRKR-like endoplasmic reticulum kinase antibody
    • WRS antibody
    see all

Anti-PERK antibody images

  • Immunofluorescence analysis of HepG2 cells using ab65142 at a 1/500-1/1000 dilution. Left image untreated. Right image treated with immunising peptide.
  • All lanes : Anti-PERK antibody (ab65142) at 1/500 dilution

    Lane 1 : Extracts from Hela cells
    Lane 2 : Extracts from HepG2 cells
    Lane 3 : Extracts from HepG2 cells, plus immunising peptide


    Predicted band size : 125 kDa
    Observed band size : 125 kDa
  • PERK was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to PERK and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65142.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) .
    Band: 130kDa: PERK.

References for Anti-PERK antibody (ab65142)

This product has been referenced in:
  • Yu Y  et al. PKR-like endoplasmic reticulum kinase is necessary for lipogenic activation during HCMV infection. PLoS Pathog 9:e1003266 (2013). WB ; Human . Read more (PubMed: 23592989) »
  • Manni S  et al. Protein kinase CK2 protects multiple myeloma cells from ER stress-induced apoptosis and from the cytotoxic effect of HSP90 inhibition through regulation of the unfolded protein response. Clin Cancer Res 18:1888-900 (2012). WB . Read more (PubMed: 22351691) »

See all 2 Publications for this product

Product Wall

Thank you for the clarification.

Have you considered using an antibody concentration kit, like our ab102776, for a buffer exchange? This may be more cost-effective than having the lab do a custom preparation.

http://www.abcam.com/in...

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Thank you for your inquiry.

The formulation for ab65142 is:

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

It does not contain any carrier prote...

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Thank you for contacting us.

Your credit note ID is XXXXXX.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used ...

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Thank you very much for your email.

We unfortunately do not have any more suggestions to make. We are not sure about the reason of non specific band so in order to understand more about the problem I am happy in providing a free of charge rep...

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Thank you for your enquiry regarding ab65142 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Additionally, thank you for supplying an image, as this has...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Murine Embryonic Fibroblast (MEF))
Specification Murine Embryonic Fibroblast (MEF)
Fixative Paraformaldehyde
Permeabilization Yes - 0,15% Triton X-100
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 21°C
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Tom Verfaillie

Verified customer

Submitted Jan 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"