Overview

  • Product name
  • Description
    Rabbit polyclonal to PERK
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the N terminal region of human PERK.

  • Positive control
    • Human hepatocarcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab77654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/1000.
WB Use at an assay dependent concentration. Predicted molecular weight: 125 kDa.

Target

  • Function
    Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.
  • Tissue specificity
    Ubiquitous. A high level expression is seen in secretory tissues.
  • Involvement in disease
    Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP.
  • Post-translational
    modifications
    Autophosphorylated.
    N-glycosylated.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781H1925 antibody
    • E2AK3_HUMAN antibody
    • EC 2.7.11.1 antibody
    • Eif2ak3 antibody
    • Eukaryotic translation initiation factor 2 alpha kinase 3 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 3 antibody
    • Heme regulated EIF2 alpha kinase antibody
    • HRI antibody
    • HsPEK antibody
    • Pancreatic eIF2 alpha kinase antibody
    • Pancreatic eIF2-alpha kinase antibody
    • PEK antibody
    • PRKR like endoplasmic reticulum kinase antibody
    • PRKR-like endoplasmic reticulum kinase antibody
    • WRS antibody
    see all

Images

  • Anti-PERK antibody (ab77654) at 1/1000 dilution + 293 cell line lysate at 35 µg

    Predicted band size : 125 kDa
  • IHC-P image of PERK staining on human cortical sections using ab77654 (1:500). The tissue was fixed in formaldehyde and the sections were then subjected to heat mediated antigen retrieval using citric acid and permeabilized using Triton-X. The sections were them blocked using 2% BSA for 2 hour at 20°C. ab77654 was diluted 1:500 and incubated with the sections for 18 hours at 4°C. The secondary antibody used was HRP conjugated Goat polyclonal to Rabbit IgG (1:500)

     

    See Abreview

  • Formalin fixed and paraffin embedded human hepatocarcinoma tissue labelled with ab77654 (at a 1/50 dilution), which was peroxidase conjugated to the secondary antibody, followed by DAB staining.

References

ab77654 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Fukomys anselli Tissue sections (Trigeminal ganglion)
Permeabilization
No
Specification
Trigeminal ganglion
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Dr. Pascal Malkemper

Verified customer

Submitted Apr 08 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Human Tissue sections (Cortical section)
Specification
Cortical section
Permeabilization
Yes - Triton-X
Fixative
Formaldehyde
Username

Dr. Martin Broadstock

Verified customer

Submitted Feb 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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