Overview

  • Product name
  • Description
    Rabbit polyclonal to PERK
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the N terminal region of human PERK.

  • Positive control
    • Human hepatocarcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab77654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/1000.
WB Use at an assay dependent concentration. Predicted molecular weight: 125 kDa.

Target

  • Function
    Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.
  • Tissue specificity
    Ubiquitous. A high level expression is seen in secretory tissues.
  • Involvement in disease
    Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP.
  • Post-translational
    modifications
    Autophosphorylated.
    N-glycosylated.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781H1925 antibody
    • E2AK3_HUMAN antibody
    • EC 2.7.11.1 antibody
    • Eif2ak3 antibody
    • Eukaryotic translation initiation factor 2 alpha kinase 3 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 3 antibody
    • Heme regulated EIF2 alpha kinase antibody
    • HRI antibody
    • HsPEK antibody
    • Pancreatic eIF2 alpha kinase antibody
    • Pancreatic eIF2-alpha kinase antibody
    • PEK antibody
    • PRKR like endoplasmic reticulum kinase antibody
    • PRKR-like endoplasmic reticulum kinase antibody
    • WRS antibody
    see all

Anti-PERK antibody images

  • Anti-PERK antibody (ab77654) at 1/1000 dilution + 293 cell line lysate at 35 µg

    Predicted band size : 125 kDa
  • IHC-P image of PERK staining on human cortical sections using ab77654 (1:500). The tissue was fixed in formaldehyde and the sections were then subjected to heat mediated antigen retrieval using citric acid and permeabilized using Triton-X. The sections were them blocked using 2% BSA for 2 hour at 20°C. ab77654 was diluted 1:500 and incubated with the sections for 18 hours at 4°C. The secondary antibody used was HRP conjugated Goat polyclonal to Rabbit IgG (1:500)

     

    See Abreview

  • Formalin fixed and paraffin embedded human hepatocarcinoma tissue labelled with ab77654 (at a 1/50 dilution), which was peroxidase conjugated to the secondary antibody, followed by DAB staining.

References for Anti-PERK antibody (ab77654)

ab77654 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Fukomys anselli Tissue sections (Trigeminal ganglion)
Permeabilization
No
Specification
Trigeminal ganglion
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Dr. Pascal Malkemper

Verified customer

Submitted Apr 08 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Human Tissue sections (Cortical section)
Specification
Cortical section
Permeabilization
Yes - Triton-X
Fixative
Formaldehyde
Username

Dr. Martin Broadstock

Verified customer

Submitted Feb 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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