Overview

  • Product nameAnti-Peroxiredoxin 1 antibody
    See all Peroxiredoxin 1 primary antibodies
  • Description
    Rabbit polyclonal to Peroxiredoxin 1
  • SpecificityThis antibody detects Peroxiredoxin 1 protein in human samples. The antibody is specific for Peroxiredoxin 1 and shows no cross reactivity with other Prx isoforms.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, African Green Monkey
    Predicted to work with: Cow, Chinese Hamster
  • Immunogen

    Synthetic peptide corresponding to Human Peroxiredoxin 1 aa 103-114.
    Sequence:

    LVSDPKRTIAQD

  • Positive control
    • TSU Pr1 cells for cell staining, human PC3 cell lysate for western blotting.

Properties

Applications

Our Abpromise guarantee covers the use of ab15571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
IHC-P Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).

Target

  • FunctionInvolved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
  • Sequence similaritiesBelongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Post-translational
    modifications
    Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heme binding 23 kDa protein antibody
    • MSP23 antibody
    • Natural killer cell-enhancing factor A antibody
    • NKEF A antibody
    • NKEF-A antibody
    • NKEFA antibody
    • OSF3 antibody
    • Osteoblast specific factor 3 antibody
    • PAG antibody
    • Paga antibody
    • PAGB antibody
    • Peroxiredoxin-1 antibody
    • PRDX1 antibody
    • PRDX1_HUMAN antibody
    • Proliferation associated gene A antibody
    • Proliferation-associated gene protein antibody
    • PRX1 antibody
    • PrxI antibody
    • TDPX2 antibody
    • Thioredoxin peroxidase 2 antibody
    • Thioredoxin-dependent peroxide reductase 2 antibody
    see all

Anti-Peroxiredoxin 1 antibody images



  • Predicted band size : 22 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Peroxiredoxin 1knockout HAP1 cell lysate (20 µg)
    Lane 3: A431 cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab15571 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab15571 was shown to recognize Peroxiredoxin 1 when Peroxiredoxin 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Peroxiredoxin 1 knockout samples were subjected to SDS-PAGE. ab15571 and ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 andGoat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • ab15571 staining Peroxiredoxin 1 in human TSU-Pr1 cells by Immunocytochemistry.
  • ICC/IF image of ab15571 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15571, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab15571 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15571, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Peroxiredoxin 1 antibody (ab15571) at 1/1000 dilution

    Lane 1 : Cell line indicated at 25 µg
    Lane 2 : As above
    Lane 3 : As above
    Lane 4 : As above
    Lane 5 : As above
    Lane 6 : As above
    Lane 7 : As above
    Lane 8 : As above
    Lane 9 : As above
    Lane 10 : As above
    Lane 11 : As above

    Secondary
    HRP-conjugated Goat anti-rabbit at 1/20000 dilution

    Predicted band size : 22 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
    Western blot analysis of Peroxiredoxin was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with ab15571 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody for at least one hour. Membranes were washed and chemiluminescent detection performed.

References for Anti-Peroxiredoxin 1 antibody (ab15571)

This product has been referenced in:

See all 10 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 50 µg
Specification Skeletal muscle
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 50 µg
Specification Skeletal muscle
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 10 2016

Application Western blot
Loading amount 20000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (Jurkats)
Specification Jurkats
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Tejpal Gill

Verified customer

Submitted Jan 13 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Rat Tissue sections (brain)
Specification brain
Permeabilization Yes - triton 0.1%
Fixative Paraformaldehyde
Username

Dr. Paul Fraser

Verified customer

Submitted Dec 16 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle (AT))
Loading amount 50 µg
Specification Skeletal muscle (AT)
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

Submitted Sep 28 2012

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit ab15571 que vous avez reçu ne fonctionne pas comme attendu.
Le numéro de commande de remplacement gratuitavec leproduit ab4...

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Thank you for your telephone enquiry yesterday.

As requested, I am forwarding a list of suitable secondary antibodies for use with ab15571 and ab6276. These have been tested and guaranteed in ICC-IF. I have selected some FITC and PE conjugat...

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We have previously been in contact regarding ab15571, which was not providing satisfactory results.

We provided some troubleshooting tips for the protocol, and I am writing to inquire if these suggestions have helped to improve the results? ...

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Thank you for your email.

I am sorry without the positive control it will be hard to determine whether the no band problem is due to antibody, protocol or target being not expressed in cell line you have been using.

We have lysates ...

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