FunctionInvolved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
Sequence similaritiesBelongs to the ahpC/TSA family. Contains 1 thioredoxin domain.
Post-translational modificationsPhosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ab59528 at 1/100 dilution staining Peroxiredoxin 1 in Rat kidney tubules by Immunofluorescence. Secondary
antibody Jackson Cy3 (1/200).
Western blot - Peroxiredoxin 1 antibody (ab59538)
All lanes : Anti-Peroxiredoxin 1 antibody (ab59538) at 1/500 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 22 kDa Observed band size : 22 kDa Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
References for Anti-Peroxiredoxin 1 antibody (ab59538)
This product has been referenced in:
Singer E et al. Stromal response to prostate cancer: nanotechnology-based detection of thioredoxin-interacting protein partners distinguishes prostate cancer associated stroma from that of benign prostatic hyperplasia. PLoS One8:e60562 (2013).
Read more (PubMed: 23762225) »
Klebe S et al. Peroxiredoxin I and II in Human Eyes: Cellular Distribution and Association with Pterygium and DNA Damage. J Histochem CytochemN/A:N/A (2013).
Read more (PubMed: 24152995) »