Overview

  • Product nameAnti-Peroxiredoxin 1 antibody
    See all Peroxiredoxin 1 primary antibodies
  • Description
    Rabbit polyclonal to Peroxiredoxin 1
  • Specificityab59538 reacts with Peroxiredoxin 1.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Cynomolgus Monkey, Chinese Hamster
  • Immunogen

    Synthetic peptide:

    PLVSDPKRTIAQDY

    , corresponding to amino acids 103-116 of Human Peroxiredoxin 1 conjugated to diphtheria toxin.

  • Positive control
    • Rat kidney and bronchiolar tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab59538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/4000. Predicted molecular weight: 22 kDa.
ELISA 1/1000 - 1/4000.
IHC-Fr 1/500 - 1/1000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.

Target

  • FunctionInvolved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
  • Sequence similaritiesBelongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Post-translational
    modifications
    Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heme binding 23 kDa protein antibody
    • MSP23 antibody
    • Natural killer cell-enhancing factor A antibody
    • NKEF A antibody
    • NKEF-A antibody
    • NKEFA antibody
    • OSF3 antibody
    • Osteoblast specific factor 3 antibody
    • PAG antibody
    • Paga antibody
    • PAGB antibody
    • Peroxiredoxin-1 antibody
    • PRDX1 antibody
    • PRDX1_HUMAN antibody
    • Proliferation associated gene A antibody
    • Proliferation-associated gene protein antibody
    • PRX1 antibody
    • PrxI antibody
    • TDPX2 antibody
    • Thioredoxin peroxidase 2 antibody
    • Thioredoxin-dependent peroxide reductase 2 antibody
    see all

Anti-Peroxiredoxin 1 antibody images

  • ab59538 at 1/100 dilution staining Peroxiredoxin 1 in Non-ciliated bronchiolar cells (Clara cells) of rat airway by Immunofluorescence. Secondary antibody Jackson Cy3 (1/200).
  • ab59528 at 1/100 dilution staining Peroxiredoxin 1 in Rat kidney tubules by Immunofluorescence. Secondary antibody Jackson Cy3 (1/200).
  • All lanes : Anti-Peroxiredoxin 1 antibody (ab59538) at 1/500 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-Peroxiredoxin 1 antibody (ab59538)

This product has been referenced in:
  • Singer E  et al. Stromal response to prostate cancer: nanotechnology-based detection of thioredoxin-interacting protein partners distinguishes prostate cancer associated stroma from that of benign prostatic hyperplasia. PLoS One 8:e60562 (2013). IHC-Fr ; Human . Read more (PubMed: 23762225) »
  • Klebe S  et al. Peroxiredoxin I and II in Human Eyes: Cellular Distribution and Association with Pterygium and DNA Damage. J Histochem Cytochem N/A:N/A (2013). IHC-P, WB ; Human . Read more (PubMed: 24152995) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Ovary)
Specification Ovary
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mrs. Suki Kang

Verified customer

Submitted May 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Kidney)
Loading amount 10 µg
Specification Kidney
Gel Running Conditions Non-reduced Denaturing (16 %)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Melanie Adler

Verified customer

Submitted Sep 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"