Anti-Peroxiredoxin 1 antibody [EPR5434] (ab109506)


  • Product nameAnti-Peroxiredoxin 1 antibody [EPR5434]
    See all Peroxiredoxin 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5434] to Peroxiredoxin 1
  • SpecificityCorresponding to residues in Human Peroxiredoxin 1
  • Tested applicationsSuitable for: WB, IP, ICC, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    ASynthetic peptide corresponding to residues near the C-terminus of Human Peroxiredoxin 1.

  • Positive control
    • WB: 293T, K562 or U87-MG cell lysate. IHC-P: Human liver or kidney tissue. IF/ICC: HeLa cell line
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab109506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 22 kDa.
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
ICC/IF 1/100.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • FunctionInvolved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
    • Sequence similaritiesBelongs to the ahpC/TSA family.
      Contains 1 thioredoxin domain.
    • Post-translational
      Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
    • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • Heme binding 23 kDa protein antibody
      • MSP23 antibody
      • Natural killer cell-enhancing factor A antibody
      • NKEF A antibody
      • NKEF-A antibody
      • NKEFA antibody
      • OSF3 antibody
      • Osteoblast specific factor 3 antibody
      • PAG antibody
      • Paga antibody
      • PAGB antibody
      • Peroxiredoxin-1 antibody
      • PRDX1 antibody
      • PRDX1_HUMAN antibody
      • Proliferation associated gene A antibody
      • Proliferation-associated gene protein antibody
      • PRX1 antibody
      • PrxI antibody
      • TDPX2 antibody
      • Thioredoxin peroxidase 2 antibody
      • Thioredoxin-dependent peroxide reductase 2 antibody
      see all

    Anti-Peroxiredoxin 1 antibody [EPR5434] images

    • Predicted band size : 22 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Peroxiredoxin knockout HAP1 cell lysate (20 µg)
      Lane 3: A431 cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109506 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab109506 was shown to specifically react with Peroxiredoxin when Peroxiredoxin knockout samples were used. Wild-type and Peroxiredoxin knockout samples were subjected to SDS-PAGE. ab109506 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-Peroxiredoxin 1 antibody [EPR5434] (ab109506) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 22 kDa
    • ICC/IF image of ab109506 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109506, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-Peroxiredoxin 1 antibody [EPR5434] (ab109506)

    ab109506 has not yet been referenced specifically in any publications.

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