Validated using a knockout cell line

Anti-Peroxiredoxin 1 antibody [EPR5434] (ab109506)


  • Product name
    Anti-Peroxiredoxin 1 antibody [EPR5434]
    See all Peroxiredoxin 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5434] to Peroxiredoxin 1
  • Specificity
    Corresponding to residues in Human Peroxiredoxin 1
  • Tested applications
    Suitable for: WB, IP, ICC, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    ASynthetic peptide corresponding to residues near the C-terminus of Human Peroxiredoxin 1.

  • Positive control
    • WB: 293T, K562 or U87-MG cell lysate. IHC-P: Human liver or kidney tissue. IF/ICC: HeLa cell line
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab109506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 22 kDa.
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
ICC/IF 1/100.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
    • Sequence similarities
      Belongs to the ahpC/TSA family.
      Contains 1 thioredoxin domain.
    • Post-translational
      Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
    • Cellular localization
      Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • Heme binding 23 kDa protein antibody
      • MSP23 antibody
      • Natural killer cell-enhancing factor A antibody
      • NKEF A antibody
      • NKEF-A antibody
      • NKEFA antibody
      • OSF3 antibody
      • Osteoblast specific factor 3 antibody
      • PAG antibody
      • Paga antibody
      • PAGB antibody
      • Peroxiredoxin-1 antibody
      • PRDX1 antibody
      • PRDX1_HUMAN antibody
      • Proliferation associated gene A antibody
      • Proliferation-associated gene protein antibody
      • PRX1 antibody
      • PrxI antibody
      • TDPX2 antibody
      • Thioredoxin peroxidase 2 antibody
      • Thioredoxin-dependent peroxide reductase 2 antibody
      see all


    • Predicted band size : 22 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Peroxiredoxin knockout HAP1 cell lysate (20 µg)
      Lane 3: A431 cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109506 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109506 was shown to specifically react with Peroxiredoxin when Peroxiredoxin knockout samples were used. Wild-type and Peroxiredoxin knockout samples were subjected to SDS-PAGE. ab109506 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • ICC/IF image of ab109506 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109506, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Peroxiredoxin 1 with unpurified ab109506 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • All lanes : Anti-Peroxiredoxin 1 antibody [EPR5434] (ab109506) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 22 kDa


    This product has been referenced in:
    • Zhang M  et al. Peroxiredoxin 1 suppresses apoptosis via regulation of the apoptosis signal-regulating kinase 1 signaling pathway in human oral leukoplakia. Oncol Lett 10:1841-1847 (2015). Read more (PubMed: 26622762) »

    See 1 Publication for this product

    Customer reviews and Q&As

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    Thank you for your interest in ab109506. We expect that this antibody will work in ICC in mouse using fluorescent detection and is guaranteed under our Abpromise. I hope this information is helpful. Please do not hesitate to contact us if you ha...

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