• Product name
    Anti-Peroxiredoxin 2 antibody
    See all Peroxiredoxin 2 primary antibodies
  • Description
    Rabbit polyclonal to Peroxiredoxin 2
  • Host species
  • Specificity
    ab59539 reacts with Peroxiredoxin 2.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Cynomolgus monkey, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 20-31 of Human Peroxiredoxin 2 conjugated to diphtheria toxin.

  • Positive control
    • Rat lung and kidney tissue.



Our Abpromise guarantee covers the use of ab59539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/4000. Predicted molecular weight: 22 kDa.
ELISA 1/1000 - 1/4000.
IHC-Fr 1/500 - 1/1000.
IHC-P Use at an assay dependent concentration.


  • Function
    Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
  • Sequence similarities
    Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis secretory sperm binding protein Li 2a antibody
    • HEL S 2a antibody
    • MGC4104 antibody
    • Natural killer cell enhancing factor B antibody
    • Natural killer cell-enhancing factor B antibody
    • Natural Killer Enhancing Factor B antibody
    • NKEF B antibody
    • NKEF-B antibody
    • NKEFB antibody
    • Peroxiredoxin-2 antibody
    • PRDX 2 antibody
    • PRDX2 antibody
    • PRDX2_HUMAN antibody
    • PrP antibody
    • PRX2 antibody
    • PRXII antibody
    • PTX1 antibody
    • TDPX1 antibody
    • Thiol Specific Antioxidant 1 antibody
    • Thiol specific antioxidant protein antibody
    • Thiol-specific antioxidant protein antibody
    • Thioredoxin Dependent Peroxide Reductase 1 antibody
    • Thioredoxin peroxidase 1 antibody
    • Thioredoxin-dependent peroxide reductase 1 antibody
    • Torin antibody
    • TPX1 antibody
    • TSA antibody
    see all


  • ICC/IF image of ab59539 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39539, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab59539 (1/2000) staining peroxiredoxin 2 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of submucosal infiltrating leukocytes.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Anti-Peroxiredoxin 2 antibody (ab59539) at 1/500 dilution + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa
    Additional bands at: 37 kDa, 49 kDa, 75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 30 seconds
  • All lanes : Anti-Peroxiredoxin 2 antibody (ab59539) at 1/5000 dilution

    All lanes : Whole tissue lysate prepared from murine liver

    Lysates/proteins at 20 µg per lane.

    All lanes : IRDye 680 conjugated goat anti-rabbit polyclonal at 1/5000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22,45 kDa (why is the actual band size different from the predicted?)

    See Abreview


This product has been referenced in:
  • Kuzuya K  et al. Proteomics analysis identified peroxiredoxin 2 involved in early-phase left ventricular impairment in hamsters with cardiomyopathy. PLoS One 13:e0192624 (2018). Read more (PubMed: 29438398) »
  • Smith NT  et al. Redox responses are preserved across muscle fibres with differential susceptibility to aging. J Proteomics 177:112-123 (2018). WB ; Mouse . Read more (PubMed: 29438851) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for your reply.

At the time of your review, we had no direct information regarding whether the product would work in mouse tissues. As such we had listed the product a 'predicted to work' based sequence homology of the immunogen and...

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Thank you for submitting your Abreview of ab59539 in mouse.

At the time of this Abreview we had not as yet tested ab59539. Thank you so much for testing this antibody in mouse.We appreciate very much your efforts and are sorry to hear that the...

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Thank you for your honest feedback. I agree that the vial of ab59539 you received does not appear to be working as expected. I am very sorry for the trouble. As per our guarantee, if this antibody has been purchased within 6 months of submitting this r...

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