Overview

  • Product nameAnti-Peroxiredoxin 2 antibody
    See all Peroxiredoxin 2 primary antibodies
  • Description
    Rabbit polyclonal to Peroxiredoxin 2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant mouse protein purified from E.coli

  • Positive control
    • HeLa, NIH3T3, C6 and A431 cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab71533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionInvolved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
  • Sequence similaritiesBelongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis secretory sperm binding protein Li 2a antibody
    • HEL S 2a antibody
    • MGC4104 antibody
    • Natural killer cell enhancing factor B antibody
    • Natural killer cell-enhancing factor B antibody
    • Natural Killer Enhancing Factor B antibody
    • NKEF B antibody
    • NKEF-B antibody
    • NKEFB antibody
    • Peroxiredoxin-2 antibody
    • PRDX 2 antibody
    • PRDX2 antibody
    • PRDX2_HUMAN antibody
    • PrP antibody
    • PRX2 antibody
    • PRXII antibody
    • PTX1 antibody
    • TDPX1 antibody
    • Thiol Specific Antioxidant 1 antibody
    • Thiol specific antioxidant protein antibody
    • Thiol-specific antioxidant protein antibody
    • Thioredoxin Dependent Peroxide Reductase 1 antibody
    • Thioredoxin peroxidase 1 antibody
    • Thioredoxin-dependent peroxide reductase 1 antibody
    • Torin antibody
    • TPX1 antibody
    • TSA antibody
    see all

Anti-Peroxiredoxin 2 antibody images

  • All lanes : Anti-Peroxiredoxin 2 antibody (ab71533) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : NIH3T3 cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : A431 cell lysate


    Predicted band size : 22 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab71533 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71533, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Peroxiredoxin 2 antibody (ab71533)

This product has been referenced in:
  • Ruggiero C  et al. Albumin-bound fatty acids but not albumin itself alter redox balance in tubular epithelial cells and induce a peroxide-mediated redox-sensitive apoptosis. Am J Physiol Renal Physiol 306:F896-906 (2014). WB ; Rat . Read more (PubMed: 24500687) »

See 1 Publication for this product

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