Overview

  • Product nameAnti-Peroxiredoxin 2 antibody [EPR5154]
    See all Peroxiredoxin 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR5154] to Peroxiredoxin 2
  • Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    corresponding to Human Peroxiredoxin 2 aa 1-100.

  • Positive control
    • 293T, HeLa, LnCaP, and SH-SY5Y cell lysates; Human prostatic hyperplasia tissue; Human stomach tissue; HeLa cells.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200 - 1/1000.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • FunctionInvolved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
  • Sequence similaritiesBelongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis secretory sperm binding protein Li 2a antibody
    • HEL S 2a antibody
    • MGC4104 antibody
    • Natural killer cell enhancing factor B antibody
    • Natural killer cell-enhancing factor B antibody
    • Natural Killer Enhancing Factor B antibody
    • NKEF B antibody
    • NKEF-B antibody
    • NKEFB antibody
    • Peroxiredoxin-2 antibody
    • PRDX 2 antibody
    • PRDX2 antibody
    • PRDX2_HUMAN antibody
    • PrP antibody
    • PRX2 antibody
    • PRXII antibody
    • PTX1 antibody
    • TDPX1 antibody
    • Thiol Specific Antioxidant 1 antibody
    • Thiol specific antioxidant protein antibody
    • Thiol-specific antioxidant protein antibody
    • Thioredoxin Dependent Peroxide Reductase 1 antibody
    • Thioredoxin peroxidase 1 antibody
    • Thioredoxin-dependent peroxide reductase 1 antibody
    • Torin antibody
    • TPX1 antibody
    • TSA antibody
    see all

Anti-Peroxiredoxin 2 antibody [EPR5154] images



  • Predicted band size : 22 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Peroxiredoxin knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: LnCaP cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab109367 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109367 was shown to specifically react with Peroxiredoxin 2 when Peroxiredoxin 2 knockout samples were used. Wild-type and Peroxiredoxin 2 knockout samples were subjected to SDS-PAGE. ab109367 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemical staining of paraffin embedded human endometrial carcinoma with purified ab109367 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunofluorescence staining of HeLa cells with purified ab109367 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab109367 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • All lanes : Anti-Peroxiredoxin 2 antibody [EPR5154] (ab109367) at 1/10000 dilution (purified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 22 kDa
    Observed band size : 22 kDa

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-Peroxiredoxin 2 antibody [EPR5154] (ab109367) at 1/10000 dilution (purified)

    Lane 1 : HEK293 whole cell lysate
    Lane 2 : LNCaP whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 22 kDa
    Observed band size : 22 kDa

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Overlay histogram showing HeLa cells fixed in 80% methanol and stained with purified ab109367 at a dilution of 1/200 (red line). The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit at a dilution of 1/500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

  • Overlay histogram showing HeLa cells stained with unpurified ab109367 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109367, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • All lanes : Anti-Peroxiredoxin 2 antibody [EPR5154] (ab109367) at 1/1000 dilution (Unpurified)

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : LnCaP cell lysate
    Lane 4 : SH-SYSY cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size : 22 kDa
  • Immunofluorescent staining of Peroxiredoxin 2 in HeLa cells using unpurified ab109367 at 1/250 dilution.

  • Immunohistochemical analysis of Peroxiredoxin 2 in paraffin-embedded Human prostatic hyperplasia tissue using unpurified ab109367 at 1/1000 dilution.

  • Immunohistochemical analysis of Peroxiredoxin 2 in paraffin-embedded Human stomach tissue using unpurified ab109367 at 1/1000 dilution.

  • Unpurified ab109367 (1/500) staining Peroxiredoxin 2 in asynchronous HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

References for Anti-Peroxiredoxin 2 antibody [EPR5154] (ab109367)

This product has been referenced in:
  • Viennois E  et al. Longitudinal study of circulating protein biomarkers in inflammatory bowel disease. J Proteomics 112:166-79 (2015). Mouse . Read more (PubMed: 25230104) »
  • Feng J  et al. Overexpression of peroxiredoxin 2 inhibits TGF-ß1-induced epithelial-mesenchymal transition and cell migration in colorectal cancer. Mol Med Rep 10:867-73 (2014). WB, IF ; Human . Read more (PubMed: 24920174) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 62.5 µg
Gel Running Conditions Reduced Denaturing (12.5% acrylamide)
Sample Pig Tissue lysate - other (Muscle sarcoplasmic extract)
Specification Muscle sarcoplasmic extract
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Shannon Cruzen

Verified customer

Submitted Feb 05 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Username

Dr. Kirk McManus

Verified customer

Submitted Sep 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"