The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: 1/50 - 1/100.
IHC-P: 1/500 - 1/1000. Antigen retrieval has been used in characterisation but
may not be necessary.
IHC-Fr: 1/500 - 1/1000.
WB: 1/1000 - 1/2000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
Sequence similaritiesBelongs to the ahpC/TSA family. Contains 1 thioredoxin domain.