Anti-Peroxiredoxin 4 antibody (ab15574)

Overview

  • Product nameAnti-Peroxiredoxin 4 antibody
    See all Peroxiredoxin 4 primary antibodies
  • Description
    Rabbit polyclonal to Peroxiredoxin 4
  • SpecificityThis antibody is specific for Peroxiredoxin 4 and shows no cross reactivity with other Peroxiredoxin isoforms.
  • Tested applicationsSuitable for: WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Human Peroxiredoxin 4. Purified human recombinant Peroxiredoxin 4 from K562 cells.

  • Positive control
    • PC3 cell lysate (WB) TSU-Pr1 cells (ICC)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 33 kDa).

Detects a band of approximately 23 kDa and 20 kDa representing Pro-Peroxiredoxin 4 and mature Peroxiredoxin 4 respectively.

ICC 1/500.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionProbably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
  • Sequence similaritiesBelongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Cellular localizationCytoplasm. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antioxidant enzyme 372 antibody
    • Antioxidant enzyme AOE372 antibody
    • AOE37 2 antibody
    • AOE37-2 antibody
    • AOE372 antibody
    • EC 1.11.1.15 antibody
    • Peroxiredoxin IV antibody
    • Peroxiredoxin-4 antibody
    • Peroxiredoxin4 antibody
    • PRDX 4 antibody
    • Prdx4 antibody
    • PRDX4_HUMAN antibody
    • PRX 4 antibody
    • Prx IV antibody
    • Prx-IV antibody
    • PRX4 antibody
    • PrxIV antibody
    • Thioredoxin dependent peroxide reductase A0372 antibody
    • Thioredoxin Peroxidase (Antioxidant Enzyme) antibody
    • Thioredoxin peroxidase antibody
    • Thioredoxin peroxidase AO372 antibody
    • Thioredoxin-dependent peroxide reductase A0372 antibody
    • TRANK antibody
    see all

Anti-Peroxiredoxin 4 antibody images

  • ab15574 staining Peroxiredoxin 4 in TSU-Pr1 cells.
  • ab15574 staining Peroxiredoxin 4 (green) in Mouse bone marrow by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and incubated with primary antibody (1/100 in 0.05% Saponin/PBS) for 1 hour at 37°C. A Cy3®-conjugated Donkey anti-rabbit IgG polyclonal (1/10000) was used as the secondary antibody.

    See Abreview

References for Anti-Peroxiredoxin 4 antibody (ab15574)

ab15574 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Bonemarrow)
Specification Bonemarrow
Fixative Paraformaldehyde
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Apr 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HELA)
Specification HELA
Fixative 3% Paraformaldehyde
Permeabilization No
Blocking step NH4Cl as blocking agent for 15 minute(s) · Concentration: 0.27% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"