Overview

  • Product nameAnti-Peroxiredoxin 4 antibody
    See all Peroxiredoxin 4 primary antibodies
  • Description
    Rabbit polyclonal to Peroxiredoxin 4
  • Specificityab59542 reacts with Peroxiredoxin 4.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Fr, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human Peroxiredoxin 4 aa 65-83.
    Sequence:

    SRVSVADHSLHLSKAKISK

  • Positive control
    • Non-ciliated bronchiolar cells (Clara cells) in rat lung and sebaceous glands in rat skin.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/4000. Predicted molecular weight: 31 kDa.
ELISA 1/1000 - 1/4000.
IHC-Fr 1/500 - 1/1000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
IP Use at an assay dependent concentration.

Target

  • FunctionProbably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
  • Sequence similaritiesBelongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Cellular localizationCytoplasm. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antioxidant enzyme 372 antibody
    • Antioxidant enzyme AOE372 antibody
    • AOE37 2 antibody
    • AOE37-2 antibody
    • AOE372 antibody
    • EC 1.11.1.15 antibody
    • Peroxiredoxin IV antibody
    • Peroxiredoxin-4 antibody
    • Peroxiredoxin4 antibody
    • PRDX 4 antibody
    • Prdx4 antibody
    • PRDX4_HUMAN antibody
    • PRX 4 antibody
    • Prx IV antibody
    • Prx-IV antibody
    • PRX4 antibody
    • PrxIV antibody
    • Thioredoxin dependent peroxide reductase A0372 antibody
    • Thioredoxin Peroxidase (Antioxidant Enzyme) antibody
    • Thioredoxin peroxidase antibody
    • Thioredoxin peroxidase AO372 antibody
    • Thioredoxin-dependent peroxide reductase A0372 antibody
    • TRANK antibody
    see all

Anti-Peroxiredoxin 4 antibody images

  • Peroxiredoxin 4 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µl of Rabbit polyclonal to Peroxiredoxin 4 sera and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab59542.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 27kDa; Peroxiredoxin 4, non specific - as present in control (lane 2); 27kDa: We are confident this was due to slight lane contamination and the band seen in the IP lane is our target of interest.

  • ab59542 at 1/100 dilution staining Peroxiredoxin 4 in sebaceous glands in rat skin by Immunohistochemistry. Secondary antibody Jackson biotinylated Donkey anti-rabbit (1/2000) followed by Vector ABC kit and visualised with DAB substrate.
  • ab59542 at 1/100 dilution staining Peroxiredoxin 4 in Non-ciliated bronchiolar cells (Clara cells) in rat lung by Immunofluorescence. Secondary antibody Jackson Cy3 (1/200).
  • All lanes : Anti-Peroxiredoxin 4 antibody (ab59542) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 74 kDa,98 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
    The band observed at 27 kDa could potentially be a cleaved form of Peroxiredoxin 4 due to the presence of a 39 amino acid signal peptide.

References for Anti-Peroxiredoxin 4 antibody (ab59542)

This product has been referenced in:
  • Jiang H  et al. Expression of peroxiredoxin 1 and 4 promotes human lung cancer malignancy. Am J Cancer Res 4:445-60 (2014). WB ; Human . Read more (PubMed: 25232487) »
  • Meng Y  et al. Downregulated expression of peroxiredoxin 4 in granulosa cells from polycystic ovary syndrome. PLoS One 8:e76460 (2013). WB, IHC-P ; Human . Read more (PubMed: 24098506) »

See all 8 Publications for this product

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The source of ab16943 informed us that theimmunogen used for this antibody isa recombinant human fragment protein (without secretion leader sequence) purified from E.coli. Only the region “WETEERPRTREEECHFYAG...

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I received some useful information from the laboratory that developed this antibody: "In human lung we see two very clean bands, one around 29kD and the other around 60kD. This protein is reported as being synthesised as a 31kD protein and a signal...

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