Western blot - Anti-Peroxiredoxin 5 antibody [12A] (ab16942)
Predicted band size : 24 kDa
Lane 1: Wild type HAP1 whole cell lysate (20 µg) Lane 2: PRDX5 knockout HAP1 whole cell lysate (20 µg) Lane 3: HepG2 whole cell lysate (20 µg) Lane 4: A549 whole cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab16942 observed at 17 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab16942 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. Ab16942 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 2000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Peroxiredoxin 5 antibody [12A] (ab16942)This image is courtesy of an anonymous Abreview
Anti-Peroxiredoxin 5 antibody [12A] (ab16942) at 1/5000 dilution + Rat cerebellum whole tissue lysate at 30 µg
Secondary Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 24 kDa Observed band size : 24 kDa