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Recombinant fragment (Human).
Our Abpromise guarantee covers the use of ab16944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 22 kDa.
Incubate the membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking overnight.
|IP||Use a concentration of 1 - 2 µg/ml.|
ab16944 (1/1000) detection of Peroxiredoxin 5 in...
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: Jurkat cell lysate