Overview

  • Product name
    Anti-Peroxiredoxin 5 antibody
    See all Peroxiredoxin 5 primary antibodies
  • Description
    Rabbit polyclonal to Peroxiredoxin 5
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cat, Dog, Pig, African green monkey
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 71-120 (TDLLLDDSLV SIFGNRRLKR FSMVVQDGIV KALNVEPDGT GLTCSLAPNI) of Human Peroxiredoxin 5 (NP_857635).

  • Positive control
    • Fetal lung lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately <22 kDa (predicted molecular weight: 22 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/12500.

Target

  • Function
    Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the peroxiredoxin 2 family.
    Contains 1 thioredoxin domain.
  • Cellular localization
    Mitochondrion. Cytoplasm. Peroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACR1 antibody
    • Alu co repressor 1 antibody
    • Alu corepressor 1 antibody
    • Antioxidant enzyme B166 antibody
    • AOEB166 antibody
    • B166 antibody
    • epididymis secretory protein Li 55 antibody
    • HEL-S-55 antibody
    • Liver tissue 2D-page spot 71B antibody
    • mitochondrial antibody
    • Peroxiredoxin 5, mitochondrial antibody
    • Peroxiredoxin V antibody
    • Peroxiredoxin-5 antibody
    • Peroxisomal antioxidant enzyme antibody
    • PLP antibody
    • PMP20 antibody
    • PRDX 5 antibody
    • PRDX5 antibody
    • PRDX5_HUMAN antibody
    • PRDX6 antibody
    • Prx-V antibody
    • PRXV antibody
    • SBBI10 antibody
    • Thioredoxin peroxidase PMP20 antibody
    • Thioredoxin reductase antibody
    • TPx type VI antibody
    see all

Images

  • Anti-Peroxiredoxin 5 antibody (ab83045) at 1 µg/ml (5% skim milk / PBS buffer) + fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 22 kDa
    Observed band size : <22 kDa (why is the actual band size different from the predicted?)

References

ab83045 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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