Overview

  • Product nameAnti-Peroxiredoxin 6 antibody
    See all Peroxiredoxin 6 primary antibodies
  • Description
    Rabbit polyclonal to Peroxiredoxin 6
  • SpecificityThis antibody is specific to the sulfinic and sulfonic form of PrxVI.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Sulfonylated peptide (KLH coupled) corresponding to the active site sequence common to mammalian Peroxiredoxin 6.

  • Positive control
    • HeLa cell lysate.

Applications

Our Abpromise guarantee covers the use of ab28444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 25 kDa.
ICC/IF Use at an assay dependent dilution. PubMed: 18826942

Target

  • FunctionInvolved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
  • Sequence similaritiesBelongs to the ahpC/TSA family. Rehydrin subfamily.
    Contains 1 thioredoxin domain.
  • Cellular localizationCytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 Cys antibody
    • 1 Cys peroxiredoxin antibody
    • 1 Cys PRX antibody
    • 1 cysPrx antibody
    • 1-Cys peroxiredoxin antibody
    • 1-Cys PRX antibody
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Anti-Peroxiredoxin 6 antibody images

  • All lanes : Anti-Peroxiredoxin 6 antibody (ab28444) at 1/2000 dilution

    Lane 1 : H2O2 untreated HeLa cell lysates
    Lane 2 : H2O2 treated HeLa cell lysates
    Lane 3 : Reduced recombinant Peroxiredoxin 6
    Lane 4 : Oxidised recombinant Peroxiredoxin 6


    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti Peroxiredoxin 6 antibody

    Lane 1 : H2O2 untreated HeLa cell lysate
    Lane 2 : H2O2 treated HeLa cell lysate
    Lane 3 : Reduced recombinant Peroxiredoxin 6
    Lane 4 : Oxidised recombinant Peroxiredoxin 6


    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

References for Anti-Peroxiredoxin 6 antibody (ab28444)

This product has been referenced in:
  • O'Flaherty C & de Souza AR Hydrogen peroxide modifies human sperm peroxiredoxins in a dose-dependent manner. Biol Reprod 84:238-47 (2011). WB ; Human . Read more (PubMed: 20864641) »
  • Kim SY  et al. H2O2-dependent hyperoxidation of peroxiredoxin 6 (Prdx6) plays a role in cellular toxicity via up-regulation of iPLA2 activity. J Biol Chem 283:33563-8 (2008). ICC/IF ; Human . Read more (PubMed: 18826942) »

See all 2 Publications for this product

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