• Product name
    Anti-Peroxiredoxin-SO3 antibody [10A1]
    See all Peroxiredoxin-SO3 primary antibodies
  • Description
    Mouse monoclonal [10A1] to Peroxiredoxin-SO3
  • Specificity
    Sulfinic and sulfonic form of Prx I to IV.
  • Tested applications
    Suitable for: ChIP, WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Sulfonylated synthetic peptide corresponding to active site of mammalian Prx I to IV conjugated to KLH.

  • Positive control
    • This antibody gave a positive result when used in the following formaldehyde fixed cell line: HeLa.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR172142 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.


Our Abpromise guarantee covers the use of ab16951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Flow Cyt Use at an assay dependent concentration.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.


  • Relevance
    Peroxiredoxin (Prx) is an antioxidant enzyme detoxifying reactive oxygen species and has a cysteine at the active site. Prx enzymes modulate various receptor signaling pathways and protect cells from oxidatively induced death. Peroxiredoxin 1 to 4 have two conserved Cys residues corresponding to Cys51 and Cys172 of mammalian Peroxiredoxin 1. The active site cysteine(Cys51) is oxidized to cysteine sulfenic acid(Cys51-SOH) when a peroxide is reduced. Because Cys51-SOH is unstable, it forms a disulfide with Cys172-SH which comes from the other subunit of the homodimer. The disulfide is then reduced back to the Prx active thiol form by the thioredoxin-thioredoxin reductase system. However, the formation of the disulfide is a slow process. Thus under oxidative stress conditions, the sulfenic intermediate(Cys51-SOH) can be easily over oxidized to cysteine sulfinic acid(Cys-SO2H) or cysteine sulfonic acid(Cys-SO3H) before it is able to form a disulfide. Recent studies suggest that over oxidized Prx can be reduced back to the active form during recovery after oxidative stress.
  • Cellular localization
  • Database links
  • Alternative names
    • Peroxiredoxin 1 antibody
    • Peroxiredoxin 2 antibody
    • Peroxiredoxin 3 antibody
    • Peroxiredoxin 4 antibody
    • PRDX1 antibody
    • PRDX2 antibody
    • PRDX3 antibody
    • PRDX4 antibody
    see all


  • ICC/IF image of ab16951 stained HeLa cells.  The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab16951 at 10 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Mouse IgG (H+L) (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab16951 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16951, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Predicted band size : 22 kDa

    WB ab16951 detection of Peroxiredoxin-SO3 in

    Lane 1: untreated HeLa cell lyate
    Lane 2: H2O2 treated HeLa cell lysate

    The predicted band appears as a doublet at 25kD. We are unsure of the identity of the upper bands.


This product has been referenced in:
  • Myers CR & Myers JM The effects of acrolein on peroxiredoxins, thioredoxins, and thioredoxin reductase in human bronchial epithelial cells. Toxicology 257:95-104 (2009). Read more (PubMed: 19135121) »

See 1 Publication for this product

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