• Product nameAnti-Peroxiredoxin-SO3 antibody [10A1]
    See all Peroxiredoxin-SO3 primary antibodies
  • Description
    Mouse monoclonal [10A1] to Peroxiredoxin-SO3
  • SpecificitySulfinic and sulfonic form of Prx I to IV.
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Sulfonylated synthetic peptide corresponding to active site of mammalian Prx I to IV conjugated to KLH.

  • Positive control
    • This antibody gave a positive result when used in the following formaldehyde fixed cell line: HeLa.



Our Abpromise guarantee covers the use of ab16951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.


  • RelevancePeroxiredoxin (Prx) is an antioxidant enzyme detoxifying reactive oxygen species and has a cysteine at the active site. Prx enzymes modulate various receptor signaling pathways and protect cells from oxidatively induced death. Peroxiredoxin 1 to 4 have two conserved Cys residues corresponding to Cys51 and Cys172 of mammalian Peroxiredoxin 1. The active site cysteine(Cys51) is oxidized to cysteine sulfenic acid(Cys51-SOH) when a peroxide is reduced. Because Cys51-SOH is unstable, it forms a disulfide with Cys172-SH which comes from the other subunit of the homodimer. The disulfide is then reduced back to the Prx active thiol form by the thioredoxin-thioredoxin reductase system. However, the formation of the disulfide is a slow process. Thus under oxidative stress conditions, the sulfenic intermediate(Cys51-SOH) can be easily over oxidized to cysteine sulfinic acid(Cys-SO2H) or cysteine sulfonic acid(Cys-SO3H) before it is able to form a disulfide. Recent studies suggest that over oxidized Prx can be reduced back to the active form during recovery after oxidative stress.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • Peroxiredoxin 1 antibody
    • Peroxiredoxin 2 antibody
    • Peroxiredoxin 3 antibody
    • Peroxiredoxin 4 antibody
    • PRDX1 antibody
    • PRDX2 antibody
    • PRDX3 antibody
    • PRDX4 antibody
    see all

Anti-Peroxiredoxin-SO3 antibody [10A1] images

  • ICC/IF image of ab16951 stained HeLa cells.  The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab16951 at 10 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Mouse IgG (H+L) (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab16951 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16951, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Predicted band size : 22 kDa

    WB ab16951 detection of Peroxiredoxin-SO3 in

    Lane 1: untreated HeLa cell lyate
    Lane 2: H2O2 treated HeLa cell lysate

    The predicted band appears as a doublet at 25kD. We are unsure of the identity of the upper bands.

References for Anti-Peroxiredoxin-SO3 antibody [10A1] (ab16951)

This product has been referenced in:
  • Myers CR & Myers JM The effects of acrolein on peroxiredoxins, thioredoxins, and thioredoxin reductase in human bronchial epithelial cells. Toxicology 257:95-104 (2009). Read more (PubMed: 19135121) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab16951.
Please use the links above to contact us or submit feedback about this product.