Overview

  • Product nameAnti-PERP antibody
    See all PERP primary antibodies
  • Description
    Goat polyclonal to PERP
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Xenopus laevis, Chimpanzee
  • Immunogen

    Synthetic peptide:

    CLPNYEDDLLGNAK

    from the C Terminus of the protein sequence (Human)

  • Positive control
    • Human Liver lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab48032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 20 kDa).
IHC-P 1/100. (see Abreview)

Target

  • FunctionComponent of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway.
  • Tissue specificityExpressed in skin, heart, placental, liver, pancreas, keratinocytes and dermal fibroblasts.
  • Sequence similaritiesBelongs to the TMEM47 family.
  • Cellular localizationCell junction > desmosome. Cell membrane. Associated with desmosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110017A08Rik antibody
    • dJ496H19.1 antibody
    • KCP 1 antibody
    • KCP-1 antibody
    • KCP1 antibody
    • Keratinocyte associated protein 1 antibody
    • Keratinocyte-associated protein 1 antibody
    • Keratinocytes associated protein 1 antibody
    • KRTCAP 1 antibody
    • KRTCAP1 antibody
    • p53 apoptosis effector related to PMP 22 antibody
    • p53 apoptosis effector related to PMP-22 antibody
    • p53 apoptosis effector related to PMP22 antibody
    • P53 induced protein PIGPC1 antibody
    • P53-induced protein PIGPC1 antibody
    • Perp antibody
    • PERP TP53 apoptosis effector antibody
    • PERP_HUMAN antibody
    • PIGPC 1 antibody
    • PIGPC1 antibody
    • RP3 496H19.1 antibody
    • THW antibody
    • TP53 apoptosis effector antibody
    • Transmembrane protein THW antibody
    see all

Anti-PERP antibody images

  • Anti-PERP antibody (ab48032) at 0.3 µg/ml + Human Liver lysate in RIPA buffer at 35 µg

    Predicted band size : 20 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
  • ab48032 staining PERP in human small intestine tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated rabbit anti-goat polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

References for Anti-PERP antibody (ab48032)

This product has been referenced in:
  • Gomez-Sanchez JC  et al. The human Tp53 Arg72Pro polymorphism explains different functional prognosis in stroke. J Exp Med 208:429-37 (2011). WB ; Human . Read more (PubMed: 21357744) »

See 1 Publication for this product

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Thank you for your email and for keeping me updated about the situation.
I'm sorry to hear that ab5986 did not work well with the mouse samples, and it is possible that this antibody just doesn't work with mouse. We have unfortunately removed ab39...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Small intestine)
Specification Small intestine
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Verified customer

Submitted May 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"