Overview

  • Product nameAnti-PERP antibody
    See all PERP primary antibodies
  • Description
    Rabbit polyclonal to PERP
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    EDDLLGNAKPRYFYTSA

    , corresponding to amino acids 175-193 of Human PERP.

  • Positive control
    • A431 whole cell lysate.
  • General notesStable for one year.


    The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in the p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesThe p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in the p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab5986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 18989386
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 21 kDa.Can be blocked with Human PERP peptide (ab39843).

Target

  • FunctionComponent of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway.
  • Tissue specificityExpressed in skin, heart, placental, liver, pancreas, keratinocytes and dermal fibroblasts.
  • Sequence similaritiesBelongs to the TMEM47 family.
  • Cellular localizationCell junction > desmosome. Cell membrane. Associated with desmosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110017A08Rik antibody
    • dJ496H19.1 antibody
    • KCP 1 antibody
    • KCP-1 antibody
    • KCP1 antibody
    • Keratinocyte associated protein 1 antibody
    • Keratinocyte-associated protein 1 antibody
    • Keratinocytes associated protein 1 antibody
    • KRTCAP 1 antibody
    • KRTCAP1 antibody
    • p53 apoptosis effector related to PMP 22 antibody
    • p53 apoptosis effector related to PMP-22 antibody
    • p53 apoptosis effector related to PMP22 antibody
    • P53 induced protein PIGPC1 antibody
    • P53-induced protein PIGPC1 antibody
    • Perp antibody
    • PERP TP53 apoptosis effector antibody
    • PERP_HUMAN antibody
    • PIGPC 1 antibody
    • PIGPC1 antibody
    • RP3 496H19.1 antibody
    • THW antibody
    • TP53 apoptosis effector antibody
    • Transmembrane protein THW antibody
    see all

Anti-PERP antibody images

  • Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with anti- PERP at 1 µg /ml.
  • Immunocytochemistry of PERP in A431 cells with PERP antibody at 10 µg/ml.

References for Anti-PERP antibody (ab5986)

This product has been referenced in:
  • Teh MT  et al. A molecular study of desmosomes identifies a desmoglein isoform switch in head and neck squamous cell carcinoma. J Oral Pathol Med 40:67-76 (2011). WB ; Human . Read more (PubMed: 20923451) »
  • Ding Z  et al. Preferential gene expression in the limbus of the vervet monkey. Mol Vis 14:2031-41 (2008). IHC-Fr ; Monkey . Read more (PubMed: 18989386) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mammary gland)
Specification Mammary gland
Fixative Acetone
Permeabilization Yes - TritonX-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted May 24 2012

Thank you for your email and for keeping me updated about the situation.
I'm sorry to hear that ab5986 did not work well with the mouse samples, and it is possible that this antibody just doesn't work with mouse. We have unfortunately removed ab39...

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Thank you for your call today and for your interest in ab48032. Please see below for the details of your testing discount code, and let me know if you have any questions or if there is anything else that we can do for you.
DISCOUNT CODE: ***
E...

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Thank you for the details that you have provided and I'm sorry to hear that you are experiencing difficulty with ab5986. At this point I would like to make the following suggestions to help improve results as you are not seeing any bands. This anti...

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I'm sorry to hear you are having a problem with ab5986. I would like to suggest an overnight incubation of the antibody at 4C and maybe try a dilution of 1:300 as well. If you still have problems and your positive control (50ug of lysate per well) c...

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Thank you for getting back to us and providing us some more information about your recent results. We can see on the Western blot image that the 21 kDa band appears nicely, however there are two other non-specific bands as well. It is important...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"