The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/32000. Peptide ELISA
Use a concentration of 2.5 - 3.8 µg/ml.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
Use at an assay dependent dilution.
FunctionComponent of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
Tissue specificitySignificant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin.
Sequence similaritiesBelongs to the pescadillo family. Contains 1 BRCT domain.
Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Chromosome. Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli.
ab19036 (3.8µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
References for Anti-Pescadillo antibody (ab19036)
This product has been referenced in:
Sikorski EM et al. Pescadillo interacts with the cadmium response element of the human heme oxygenase-1 promoter in renal epithelial cells. J Biol Chem281:24423-30 (2006).
Read more (PubMed: 16816389) »