Overview

  • Product nameAnti-Pescadillo antibody
    See all Pescadillo primary antibodies
  • Description
    Goat polyclonal to Pescadillo
  • Tested applicationsSuitable for: ELISA, ICC, WB, EMSAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Pig
  • Immunogen

    Synthetic peptide:

    GGLEKKKYERGSA

    , corresponding to N terminal amino acids 2-14 of Human Pescadillo.

  • Positive control
    • Hela lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab19036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/32000. Peptide ELISA
ICC Use a concentration of 2.5 - 3.8 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
EMSA Use at an assay dependent dilution.

Target

  • FunctionComponent of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
  • Tissue specificitySignificant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin.
  • Sequence similaritiesBelongs to the pescadillo family.
    Contains 1 BRCT domain.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Chromosome. Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ43790 antibody
    • Homo sapiens pescadillo mRNA complete cds antibody
    • PES antibody
    • PES 1 antibody
    • pes1 antibody
    • PESC_HUMAN antibody
    • Pescadillo (zebrafish) homolog 1 containing BRCT domain antibody
    • Pescadillo homolog 1 antibody
    • Pescadillo homolog 1 containing BRCT domain (zebrafish) antibody
    • Pescadillo homolog antibody
    • Pescadillo ribosomal biogenesis factor 1 antibody
    see all

Anti-Pescadillo antibody images



  • Predicted band size : 68 kDa


    ab19036 (1µg/ml) staining of Hela lysate (35µg protein in RIPA buffer) approx. 70kDa. Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab19036 (3.8µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References for Anti-Pescadillo antibody (ab19036)

This product has been referenced in:
  • Sikorski EM  et al. Pescadillo interacts with the cadmium response element of the human heme oxygenase-1 promoter in renal epithelial cells. J Biol Chem 281:24423-30 (2006). Read more (PubMed: 16816389) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"