Overview

  • Product name
  • Description
    Rabbit polyclonal to Pescadillo
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 535 and the C-terminus (residue 588) of human Pescadillo (NP_055118.1)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab72539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
  • Tissue specificity
    Significant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin.
  • Sequence similarities
    Belongs to the pescadillo family.
    Contains 1 BRCT domain.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localization
    Nucleus > nucleolus. Nucleus > nucleoplasm. Chromosome. Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ43790 antibody
    • Homo sapiens pescadillo mRNA complete cds antibody
    • PES antibody
    • PES 1 antibody
    • pes1 antibody
    • PESC_HUMAN antibody
    • Pescadillo (zebrafish) homolog 1 containing BRCT domain antibody
    • Pescadillo homolog 1 antibody
    • Pescadillo homolog 1 containing BRCT domain (zebrafish) antibody
    • Pescadillo homolog antibody
    • Pescadillo ribosomal biogenesis factor 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Pescadillo with ab72539 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-Pescadillo antibody (ab72539) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human Pescadillo by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72539 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

References

ab72539 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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