The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
Use at 2-5 µg/mg of lysate.
FunctionComponent of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
Tissue specificitySignificant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin.
Sequence similaritiesBelongs to the pescadillo family. Contains 1 BRCT domain.
Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Chromosome. Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli.
Detection of Human Pescadillo by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72539 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
References for Anti-Pescadillo antibody (ab72539)
has not yet been referenced specifically in any publications.
Publishing research using ab72539? Please let us know so that we can cite the reference in this datasheet.
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