Anti-Pescadillo antibody (ab88543)
Key features and details
- Mouse polyclonal to Pescadillo
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Pescadillo antibody
See all Pescadillo primary antibodies -
Description
Mouse polyclonal to Pescadillo -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein within Human Pescadillo. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: PC-12 and A-431 cell lysates; Pescadillo transfected 293T cell lysate ICC/IF: HeLa cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. -
Tissue specificity
Significant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin. -
Sequence similarities
Belongs to the pescadillo family.
Contains 1 BRCT domain. -
Post-translational
modificationsSumoylated. -
Cellular localization
Nucleus > nucleolus. Nucleus > nucleoplasm. Chromosome. Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli. - Information by UniProt
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Database links
- Entrez Gene: 23481 Human
- Entrez Gene: 289740 Rat
- Omim: 605819 Human
- SwissProt: O00541 Human
- SwissProt: Q3B8N8 Rat
- Unigene: 517543 Human
- Unigene: 9521 Rat
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Alternative names
- FLJ43790 antibody
- Homo sapiens pescadillo mRNA complete cds antibody
- PES antibody
see all
Images
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Anti-Pescadillo antibody (ab88543) at 1 µg/ml + PC-12 cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
Anti-Pescadillo antibody (ab88543) at 1 µg/ml + A-431 cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 68 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Pescadillo antibody (ab88543) at 1 µg/ml
Lane 1 : Pescadillo transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
ab88543, at 10 µg/ml, staining Pescadillo in HeLa cell by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab88543 has been referenced in 3 publications.
- Xu KD et al. Licochalcone A inhibits cell growth through the downregulation of the Hippo pathway via PES1 in cholangiocarcinoma cells. Environ Toxicol 37:564-573 (2022). PubMed: 34845814
- Chen B et al. The Long Noncoding RNA CCAT2 Induces Chromosomal Instability Through BOP1-AURKB Signaling. Gastroenterology 159:2146-2162.e33 (2020). PubMed: 32805281
- Nakaguro M et al. Nucleolar protein PES1 is a marker of neuroblastoma outcome and is associated with neuroblastoma differentiation. Cancer Sci 106:237-43 (2015). ICC . PubMed: 25557119