Overview

  • Product name
  • Description
    Mouse polyclonal to Pescadillo
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length human Pescadillo protein (AAH32489.1).

  • Positive control
    • WB: PC-12 and A-431 cell lysates; Pescadillo transfected 293T cell lysate ICC/IF: HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab88543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
  • Tissue specificity
    Significant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin.
  • Sequence similarities
    Belongs to the pescadillo family.
    Contains 1 BRCT domain.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localization
    Nucleus > nucleolus. Nucleus > nucleoplasm. Chromosome. Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ43790 antibody
    • Homo sapiens pescadillo mRNA complete cds antibody
    • PES antibody
    • PES 1 antibody
    • pes1 antibody
    • PESC_HUMAN antibody
    • Pescadillo (zebrafish) homolog 1 containing BRCT domain antibody
    • Pescadillo homolog 1 antibody
    • Pescadillo homolog 1 containing BRCT domain (zebrafish) antibody
    • Pescadillo homolog antibody
    • Pescadillo ribosomal biogenesis factor 1 antibody
    see all

Images

  • Anti-Pescadillo antibody (ab88543) at 1 µg/ml + PC-12 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 68 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Anti-Pescadillo antibody (ab88543) at 1 µg/ml + A-431 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 68 kDa
    Observed band size : 73 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Pescadillo antibody (ab88543) at 1 µg/ml

    Lane 1 : Pescadillo transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 68 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • ab88543, at 10 µg/ml, staining Pescadillo in HeLa cell by Immunofluorescence.

References

This product has been referenced in:
  • Nakaguro M  et al. Nucleolar protein PES1 is a marker of neuroblastoma outcome and is associated with neuroblastoma differentiation. Cancer Sci 106:237-43 (2015). ICC . Read more (PubMed: 25557119) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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