Pescadillo overexpression 293T lysate (whole cell) (ab94282)

Overview

  • Product namePescadillo overexpression 293T lysate (whole cell)
    See all Pescadillo lysates
  • Description
    Pescadillo overexpression lysate
  • General notesab94282 is a 293T cell transfected lysate in which Human Pescadillo has been transiently over-expressed using a pCMV-Pescadillo plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. Tissue specificity: Significant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin. Similarity: Belongs to the pescadillo family. Contains 1 BRCT domain. PTM: Sumoylated.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Pescadillo overexpression 293T lysate (whole cell) images

  • ab94282 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Pescadillo antibody (ab88543) at 1/500 dilution

    Lane 1 : Pescadillo overexpression 293T lysate (whole cell) (ab94282)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Pescadillo overexpression 293T lysate (whole cell) (ab94282)

ab94282 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Questions for ab94282.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"