Overview

  • Product name
  • Description
    Rabbit polyclonal to PET112L
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: WKREGKTPGQ IVSEKQLELM QDQGALEQLC HSVMEAHPQV VMDVKNRNPR AINKLIGLVR KATQSRADPV MIKEILEKK, corresponding to amino acids of Human PET112L.

  • Positive control
    • RT4, U251 MG, Human liver and tonsil lysates; Human colon tissue; U 2 OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab122802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.

Target

  • Function
    Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
  • Tissue specificity
    Predominantly expressed in tissues characterized by high rates of oxidative phosphorylation (OxPhos), including muscle and heart.
  • Sequence similarities
    Belongs to the gatB/gatE family. GatB subfamily.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytochrome c oxidase assembly factor PET112 homolog antibody
    • Cytochrome oxidase assembly factor PET112 homolog antibody
    • GATB_HUMAN antibody
    • glu ADT subunit B antibody
    • GLU-ADT subunit B antibody
    • glutamyl tRNA Gln amidotransferase subunit B mitochondrial antibody
    • glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial antibody
    • HSPC199 antibody
    • mitochondrial antibody
    • PET112 antibody
    • PET112 homolog (yeast) antibody
    • PET112-like antibody
    • Probable glutamyl-tRNA(Gln) amidotransferase subunit B antibody
    see all

Images

  • All lanes : Anti-PET112L antibody (ab122802) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique
  • ab122802, at 1/500 dilution, staining PET112L in Paraffin-embedded Human colon tissue by Immunohistochemistry
  • ab122802 at 4 µg/ml staining PET112L in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).

References

ab122802 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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