The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500.
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
FunctionFurnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
Tissue specificityPredominantly expressed in tissues characterized by high rates of oxidative phosphorylation (OxPhos), including muscle and heart.
Sequence similaritiesBelongs to the gatB/gatE family. GatB subfamily.