Overview

  • Product nameAnti-PEX5 antibody [6E9]
    See all PEX5 primary antibodies
  • Description
    Mouse monoclonal [6E9] to PEX5
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant full length Human PEX5 produced in HEK293T cells (NP_000310).

  • Positive control
    • pCMV6-ENTRY PEX5-transfected HEK293T cells and lysate; HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, and MCF7 cell extracts; COS7 cells transiently transfected by pCMV6-ENTRY PEX5.
  • General notesDilute in PBS (pH7.3) before use.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab125689 is purified from Mouse ascites fluid by affinity chromatography.
  • ClonalityMonoclonal
  • Clone number6E9
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 71 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionBinds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import.
  • Tissue specificityDetected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Involvement in diseaseDefects in PEX5 are a cause of adrenoleukodystrophy neonatal (NALD) [MIM:202370]. NALD is a peroxisome biogenesis disorder (PBD) characterized by the accumulation of very long-chain fatty acids, adrenal insufficiency and mental retardation. Inheritance is autosomal recessive.
    Defects in PEX5 are a cause of Zellweger syndrome (ZWS) [MIM:214100]. ZWS is a fatal peroxisome biogenesis disorder characterized by dysmorphic facial features, hepatomegaly, ocular abnormalities, renal cysts, hearing impairment, profound psychomotor retardation, severe hypotonia and neonatal seizures. Death occurs within the first year of life.
    Defects in PEX5 may be a cause of infantile Refsum disease (IRD) [MIM:266510]. IRD is a mild peroxisome biogenesis disorder (PBD). Clinical features include early onset, mental retardation, minor facial dysmorphism, retinopathy, sensorineural hearing deficit, hepatomegaly, osteoporosis, failure to thrive, and hypocholesterolemia. The biochemical abnormalities include accumulation of phytanic acid, very long chain fatty acids (VLCFA), di- and trihydroxycholestanoic acid and pipecolic acid.
  • Sequence similaritiesBelongs to the peroxisomal targeting signal receptor family.
    Contains 7 TPR repeats.
  • Cellular localizationCytoplasm. Peroxisome membrane. Its distribution appears to be dynamic. It is probably a cycling receptor found mainly in the cytoplasm and as well associated to the peroxisomal membrane through a docking factor.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ50634 antibody
    • FLJ50721 antibody
    • FLJ51948 antibody
    • PBD2A antibody
    • PBD2B antibody
    • Peroxin 5 antibody
    • Peroxin-5 antibody
    • Peroxisomal biogenesis factor 5 antibody
    • Peroxisomal C terminal targeting signal import receptor antibody
    • Peroxisomal C-terminal targeting signal import receptor antibody
    • Peroxisomal targeting signal 1 (SKL type) receptor antibody
    • Peroxisomal targeting signal 1 receptor antibody
    • Peroxisomal targeting signal import receptor antibody
    • Peroxisomal targeting signal receptor 1 antibody
    • Peroxisome receptor 1 antibody
    • pex5 antibody
    • PEX5_HUMAN antibody
    • PTS1 BP antibody
    • PTS1 receptor antibody
    • PTS1-BP antibody
    • PTS1R antibody
    • PXR1 antibody
    see all

Anti-PEX5 antibody [6E9] images

  • All lanes : Anti-PEX5 antibody [6E9] (ab125689) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PEX5 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 71 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202062)
  • All lanes : Anti-PEX5 antibody [6E9] (ab125689) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 71 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202062)
  • ab125689 at 1/100 dilution staining PEX5 in COS7 cells transiently transfected with pCMV6-ENTRY PEX5 by Immunofluorescence.
  • ab125689 at 1/100 dilution staining PEX5 in HEK293T cells transfected with either pCMV6-ENTRY PEX5 (Red) or empty vector control plasmid (Blue) by Flow Cytometry.

References for Anti-PEX5 antibody [6E9] (ab125689)

ab125689 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"