Overview

  • Product name
  • Description
    Rabbit polyclonal to PF4
  • Tested applications
    Suitable for: WB, ELISA, Neutralising, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hPF-4 (human Platelet Factor-4)

Properties

Applications

Our Abpromise guarantee covers the use of ab9561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hPF-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPF-4.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hPF-4 (50 ng/ml), a concentration of 1.75 - 3.0 µg/ml of this antibody is required.
IHC-P 1/100.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to PF4 (Biotin) (ab83123).

Can be paired for Sandwich ELISA with ab83123

Target

  • Function
    Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Binds non-covalently to a proteoglycan molecule.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C motif chemokine 4 antibody
    • Chemokine (C X C motif) ligand 4 antibody
    • Chemokine (CXC motif) ligand 4 antibody
    • CXCL 4 antibody
    • CXCL4 antibody
    • Iroplact antibody
    • MGC138298 antibody
    • Oncostatin A antibody
    • Oncostatin-A antibody
    • OncostatinA antibody
    • PF 4 antibody
    • PF-4 antibody
    • PF4 antibody
    • Platelet factor 4 antibody
    • PLF4_HUMAN antibody
    • SCYB 4 antibody
    • SCYB4 antibody
    • short form antibody
    • Small inducible cytokine subfamily member 4 antibody
    see all

Anti-PF4 antibody images

  • Anti-PF4 antibody (ab9561) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 11 kDa
    Observed band size : 11 kDa
    Additional bands at : 18 kDa,30 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-PF4 antibody (ab9561)

This product has been referenced in:
  • Izumi M  et al. Reduced induction of anti-PF4/heparin antibody in RA patients after total knee arthroplasty. Arthritis Res Ther 18:191 (2016). Human . Read more (PubMed: 27558507) »
  • Du Y  et al. Wnt3a is critical for endothelial progenitor cell-mediated neural stem cell proliferation and differentiation. Mol Med Rep 14:2473-82 (2016). WB . Read more (PubMed: 27484039) »

See all 5 Publications for this product

Product Wall

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Ab9561 has been used in neutralizing assays and has been published byLambert MPet al.Platelet factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic implications.Blood110...

Read More
Application
Western blot
Sample
Mouse Tissue lysate - other (Platelet lysate)
Loading amount
50 µg
Specification
Platelet lysate
Gel Running Conditions
Reduced Denaturing (4-15% Tris Hcl gel)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Submitted Aug 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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