Overview

  • Product name
    Anti-PF4 antibody [RTO]
    See all PF4 primary antibodies
  • Description
    Mouse monoclonal [RTO] to PF4
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IF, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Does not react with: Mouse
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysate: Human Platelet
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab49735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 11 kDa.
Flow Cyt Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 50 µg/ml.
ELISA Use a concentration of 0.1 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Binds non-covalently to a proteoglycan molecule.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C motif chemokine 4 antibody
    • Chemokine (C X C motif) ligand 4 antibody
    • Chemokine (CXC motif) ligand 4 antibody
    • CXCL 4 antibody
    • CXCL4 antibody
    • Iroplact antibody
    • MGC138298 antibody
    • Oncostatin A antibody
    • Oncostatin-A antibody
    • OncostatinA antibody
    • PF 4 antibody
    • PF-4 antibody
    • PF4 antibody
    • Platelet factor 4 antibody
    • PLF4_HUMAN antibody
    • SCYB 4 antibody
    • SCYB4 antibody
    • short form antibody
    • Small inducible cytokine subfamily member 4 antibody
    see all

Images

  • Anti-PF4 antibody [RTO] (ab49735) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 11 kDa
    Observed band size : 11 kDa


    Exposure time : 20 minutes

References

This product has been referenced in:
  • Zabini D  et al. Angiostatic factors in the pulmonary endarterectomy material from chronic thromboembolic pulmonary hypertension patients cause endothelial dysfunction. PLoS One 7:e43793 (2012). IHC . Read more (PubMed: 22916307) »
  • Li Y  et al. Elevated expression of CXC chemokines in pediatric osteosarcoma patients. Cancer 117:207-17 (2011). IHC ; Human . Read more (PubMed: 20806347) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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