Overview

  • Product namePF4 (CXCL4) Mouse ELISA KitSee all PF4 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 73.99 67% - 83%
    Serum 118.2 104% - 127%
    Plasma 109.3 92% - 129%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s PF4 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse PF4 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse PF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and PF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse PF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionReleased during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Binds non-covalently to a proteoglycan molecule.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • C X C motif chemokine 4
    • C-X-C motif chemokine 4
    • Chemokine (C X C motif) ligand 4
    • Chemokine (CXC motif) ligand 4
    • Chemokine, CXC motif, ligand 4
    • CXCL 4
    • CXCL4
    • Iroplact
    • MGC138298
    • Oncostatin A
    • Oncostatin-A
    • OncostatinA
    • PF 4
    • PF-4
    • PF4
    • Pf4a
    • Platelet factor 4
    • PLF4_HUMAN
    • RATPF4A
    • SCYB 4
    • SCYB4
    • short form
    • Small inducible cytokine subfamily B, member 4
    • Small inducible cytokine subfamily member 4
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

PF4 (CXCL4) Mouse ELISA Kit images

  • Representative standard curve using ab100735

  • Representative standard curve using ab100735

Protocols

References for PF4 (CXCL4) Mouse ELISA Kit (ab100735)

This product has been referenced in:
  • Petzold T  et al. ß1 integrin-mediated signals are required for platelet granule secretion and hemostasis in mouse. Blood 122:2723-31 (2013). Mouse . Read more (PubMed: 24004668) »

See 1 Publication for this product

Product Wall


The lab let me know that sodium citrate is also an acceptable anti-coagulant.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"