Overview

  • Product nameAnti-PFAS antibody
    See all PFAS primary antibodies
  • Description
    Rabbit polyclonal to PFAS
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Guinea pig, Cat
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 252-301 (ESIMSTQESS NPNNVLKFCD NSSAIQGKEV RFLRPEDPTR PSRFQQQQGL) of Human PFAS (NP_036525).

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 145 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevancePurines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. PFAS catalyzes the fourth step of IMP biosynthesis.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • PFAS antibody
    • FGAM synthase antibody
    • FGAMS antibody
    • FGAR amidotransferase antibody
    • FGARAT antibody
    • Formylglycinamide ribotide amidotransferase antibody
    • Formylglycinamide ribotide synthetase antibody
    • KIAA0361 antibody
    • Phosphoribosylformylglycinamidine synthase antibody
    • PURL antibody
    see all

Anti-PFAS antibody images

  • Anti-PFAS antibody (ab82755) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 145 kDa
    Observed band size : 145 kDa
  • All lanes : Anti-PFAS antibody (ab82755) at 1/2000 dilution

    Lane 1 : Human Skin Fibroblasts at 100 µg
    Lane 2 : HEK273 cells at 20 µg
    Lane 3 : HeLa cells at 20 µg


    Predicted band size : 145 kDa

References for Anti-PFAS antibody (ab82755)

This product has been referenced in:
  • French JB  et al. Hsp70/Hsp90 chaperone machinery is involved in the assembly of the purinosome. Proc Natl Acad Sci U S A 110:2528-33 (2013). Read more (PubMed: 23359685) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"