The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/1000.
WB: 1/50 - 1/100. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). Also detects a range of bands 28-72kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionBinds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
Sequence similaritiesBelongs to the prefoldin subunit beta family.