Anti-PFKFB1 antibody (ab154573)
Key features and details
- Rabbit polyclonal to PFKFB1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PFKFB1 antibody
See all PFKFB1 primary antibodies -
Description
Rabbit polyclonal to PFKFB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Pig -
Immunogen
Recombinant fragment, corresponding to a region within the amino acids 1-194 of Human PFKFB1 (Uniprot ID: P16118).
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Positive control
- H1299 & HeLa whole cell lysate, HeLa cells, H358 xenograft, 293T, MOLT4, Raji.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 55 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Synthesis and degradation of fructose 2,6-bisphosphate. -
Tissue specificity
Liver. -
Sequence similarities
In the C-terminal section; belongs to the phosphoglycerate mutase family. - Information by UniProt
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Database links
- Entrez Gene: 5207 Human
- Entrez Gene: 5208 Human
- Entrez Gene: 5209 Human
- Entrez Gene: 5210 Human
- Entrez Gene: 117276 Rat
- Entrez Gene: 24638 Rat
- Entrez Gene: 24640 Rat
- Entrez Gene: 54283 Rat
see all -
Alternative names
- 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 1 antibody
- 6-bisphosphatase antibody
- 6-P2ase 1 antibody
see all
Images
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All lanes : Anti-PFKFB1 antibody (ab154573) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : Hela whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa
10% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells labeling PFKFB1 with ab154573 at 1/200 dilution (Green). Lower panel co-stained with Hoechst 33342 (blue).
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Immunohistochemical analysis of paraffin-embedded H358 xenograft labeling PFKFB1 with ab154573 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154573 has not yet been referenced specifically in any publications.