Overview

  • Product name
  • Description
    Rabbit polyclonal to PFKFB1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within the amino acids 1-194 of Human PFKFB1 (Uniprot ID: P16118).

  • Positive control
    • H1299 & HeLa whole cell lysate, HeLa cells, H358 xenograft, 293T, MOLT4, Raji.

Properties

Applications

Our Abpromise guarantee covers the use of ab154573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-PFKFB1 antibody (ab154573) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : Hela whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 55 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells labeling PFKFB1 with ab154573 at 1/200 dilution (Green). Lower panel co-stained with Hoechst 33342 (blue).
  • Immunohistochemical analysis of paraffin-embedded H358 xenograft labeling PFKFB1 with ab154573 at 1/500 dilution.

References

ab154573 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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