Overview

  • Product name
  • Description
    Rabbit polyclonal to PFKFB1
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the center region of human PFKFB1 conjugated to KLH

  • Positive control
    • Jurkat cell line lysate and human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab71625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa).

Target

Anti-PFKFB1 antibody images

  • Anti-PFKFB1 antibody (ab71625) at 1/240 dilution + Jurkat cell line lysate at 35 µg

    Predicted band size : 55 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • ab71625, at 1/50 dilution, staining PFKFB1 in formalin fixed, paraffin embedded human hepatocarcinoma tissue by Immunohistochemistry. ab71625 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

References for Anti-PFKFB1 antibody (ab71625)

This product has been referenced in:
  • Storm P  et al. Conserved features of cancer cells define their sensitivity to HAMLET-induced death; c-Myc and glycolysis. Oncogene 30:4765-79 (2011). WB . Read more (PubMed: 21643007) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
15 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing ('any Kd' Biorad precast)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Christoph Schmitt

Verified customer

Submitted Jul 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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