Overview

  • Product nameAnti-PFKFB1 antibody
    See all PFKFB1 primary antibodies
  • Description
    Rabbit polyclonal to PFKFB1
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the center region of human PFKFB1 conjugated to KLH

  • Positive control
    • Jurkat cell line lysate and human hepatocarcinoma tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab71625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa).

Target

Anti-PFKFB1 antibody images

  • Anti-PFKFB1 antibody (ab71625) at 1/240 dilution + Jurkat cell line lysate at 35 µg

    Predicted band size : 55 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • ab71625, at 1/50 dilution, staining PFKFB1 in formalin fixed, paraffin embedded human hepatocarcinoma tissue by Immunohistochemistry. ab71625 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

References for Anti-PFKFB1 antibody (ab71625)

This product has been referenced in:
  • Storm P  et al. Conserved features of cancer cells define their sensitivity to HAMLET-induced death; c-Myc and glycolysis. Oncogene 30:4765-79 (2011). WB . Read more (PubMed: 21643007) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 15 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing ('any Kd' Biorad precast)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Christoph Schmitt

Verified customer

Submitted Jul 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"