Anti-PFKFB1 antibody (ab72590)

Overview

  • Product nameAnti-PFKFB1 antibody
    See all PFKFB1 primary antibodies
  • Description
    Rabbit polyclonal to PFKFB1
  • SpecificityThis antibody detects PFKFB1/2/4, but not PFKFB3.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human PFKFB1.

  • Positive control
    • Extracts from HeLa cells, HepG2 cells, COLO205 cells and 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ELISA 1/20000.

Target

Anti-PFKFB1 antibody images

  • All lanes : Anti-PFKFB1 antibody (ab72590) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from HepG2 cells
    Lane 3 : Extracts from COLO205 cells
    Lane 4 : Extracts from 293 cells
    Lane 5 : Extracts from 293 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 70 kDa,73 kDa. We are unsure as to the identity of these extra bands.

References for Anti-PFKFB1 antibody (ab72590)

ab72590 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply and for sending your WB image.

Perhaps that band is PFKFB4 isoform 3? That isoform is 413 a.a. long and is 47.6 kDa.

http://www.uniprot.org/blast/?about=Q6DTY7-3

I hope this information helps. Please ...

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Thank you for your inquiry.

This antibody hasn't been tested in mouse, so it is difficult to tell you for certain what the expected band pattern is. On top of that PFKFB1/2/4 have many isoforms with MW ranging from 25 - 52 kDa.

http:/...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116)
Loading amount 25 µg
Specification HCT116
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Nov 23 2010

Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount 25 µg
Specification NIH 3T3 cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"