Anti-PFKFB1 antibody (ab72590)

Overview

  • Product name
  • Description
    Rabbit polyclonal to PFKFB1
  • Specificity
    This antibody detects PFKFB1/2/4, but not PFKFB3.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human PFKFB1.

  • Positive control
    • Extracts from HeLa cells, HepG2 cells, COLO205 cells and 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ELISA 1/20000.

Target

Images

  • All lanes : Anti-PFKFB1 antibody (ab72590) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from HepG2 cells
    Lane 3 : Extracts from COLO205 cells
    Lane 4 : Extracts from 293 cells
    Lane 5 : Extracts from 293 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 70 kDa,73 kDa. We are unsure as to the identity of these extra bands.

References

ab72590 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your reply and for sending your WB image.

Perhaps that band is PFKFB4 isoform 3? That isoform is 413 a.a. long and is 47.6 kDa.

http://www.uniprot.org/blast/?about=Q6DTY7-3

I hope this information helps. Please ...

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Thank you for your inquiry.

This antibody hasn't been tested in mouse, so it is difficult to tell you for certain what the expected band pattern is. On top of that PFKFB1/2/4 have many isoforms with MW ranging from 25 - 52 kDa.

http:/...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116)
Loading amount
25 µg
Specification
HCT116
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 23 2010

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount
25 µg
Specification
NIH 3T3 cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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