Anti-PFKFB2 antibody (ab70175)

Overview

  • Product nameAnti-PFKFB2 antibody
    See all PFKFB2 primary antibodies
  • Description
    Rabbit polyclonal to PFKFB2
  • SpecificityThis antibody detects endogenous levels of total PFKFB2 protein.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthesized peptide derived from the N-terminal of human PFKFB2.

  • Positive control
    • Extracts from Jurkat cells This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2

Properties

Applications

Our Abpromise guarantee covers the use of ab70175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 58 kDa.
ELISA 1/10000.
ICC/IF Use a concentration of 5 µg/ml.

Target

Anti-PFKFB2 antibody images

  • ICC/IF image of ab70175 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab70175 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-PFKFB2 antibody (ab70175) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Various other bands are detected, from 26 to over 117kDa.
  • Anti-PFKFB2 antibody (ab70175) at 1/500 dilution (in 5% Marvel TBS-T for 24 hours at 22°C) + Whole cell lysate of mouse NIH 3T£ cells at 25 µg

    Secondary
    An HRP-conjugated Sheep anti-rabbit monoclonal at 1/5000 dilution

    Predicted band size : 58 kDa


    Exposure time : 8 minutes

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% BSA for 1 hour at 22°C

    See Abreview

References for Anti-PFKFB2 antibody (ab70175)

ab70175 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Prostate)
Gel Running Conditions Reduced Denaturing
Loading amount 60 µg
Specification Prostate
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

Submitted Nov 10 2015

Je vous recommanderais ab70175 plutôt qu’ab135821 car ab70175 est déjà garanti de fonctionner chez la souris.

Pour ab135821 bien que nous avons eu un retour positif d’un de nos clients (http://www.abcam.com/PFKF...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT 116 cells)
Loading amount 25 µg
Specification HCT 116 cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 23 2010

Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount 25 µg
Specification NIH 3T3 cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"