Overview

  • Product name
  • Description
    Rabbit polyclonal to PFKL
  • Specificity
    This antibody is specific for Fructose 6 Phosphate Kinase Liver type (isoenzyme B).
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide selected within aa 650~700 of human PFKL (C-Terminal).

  • Positive control
    • HepG2 cell lysate and hepatocarcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab37583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IHC-P 1/50 - 1/100.

Target

  • Relevance
    PFKL is the Liver isozyme of PFK (Phosphofructokinase), which is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • PFK liver isoenzyme antibody
    • 6 phosphofructokinase, liver type antibody
    • K6PL antibody
    • liver phosphofructokinase antibody
    • liver type 1 phosphofructokinase antibody
    • PFK B antibody
    • PFK liver isozyme antibody
    • Phosphofructo 1 kinase isozyme B antibody
    • Phosphofructokinase 1 antibody
    • Phosphofructokinase, liver antibody
    • Phosphohexokinase antibody
    see all

Anti-PFKL antibody images

  • Anti-PFKL antibody (ab37583) at 1/100 dilution + HepG2 cell lysate

    Predicted band size : 85 kDa
    Observed band size : 85 kDa
  • Staining of Fructose 6 Phosphate Kinase on Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue, using ab37583 at a 1/50 dilution (a peroxidase-conjugated the secondary antibody, followed by AEC staining was used).

    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

References for Anti-PFKL antibody (ab37583)

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5%
Sample
Human Cell (A549 Lung cancer cells)
Specification
A549 Lung cancer cells
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

Submitted Mar 11 2015

Thank you for contacting us. This antibody has been tested in WB with human samples and is therefore guaranteed for this application and species. I am not sure if fibroblasts specifically have been tested, but if the protein is expressed in this cell...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (HeLa (cervical cancer cells))
Loading amount
25 µg
Specification
HeLa (cervical cancer cells)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mrs. Amy Clem

Verified customer

Submitted Mar 30 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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