Overview

  • Product nameAnti-PFKP antibody
    See all PFKP primary antibodies
  • Description
    Mouse polyclonal to PFKP
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1 - 784 of Human PFKP (NP_002618.1)

  • Positive control
    • WB: pancreas tissue lysate or Hela nuclear extracts; ICC/IF: Hela cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab89003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
  • Sequence similaritiesBelongs to the phosphofructokinase family. Two domains subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1200015H23Rik antibody
    • 6 phosphofructokinase type C antibody
    • 6 phosphofructokinase, platelet type antibody
    • 6-phosphofructokinase antibody
    • 6-phosphofructokinase type C antibody
    • 9330125N24Rik antibody
    • FLJ40226 antibody
    • K6PP antibody
    • K6PP_HUMAN antibody
    • MGC105718 antibody
    • PFK C antibody
    • PFK, fibroblast type antibody
    • PFK-C antibody
    • PFKF antibody
    • PFKP antibody
    • Phosphofructo 1 kinase isozyme C antibody
    • Phosphofructo-1-kinase isozyme C antibody
    • Phosphofructokinase 1 antibody
    • Phosphofructokinase platelet antibody
    • Phosphohexokinase antibody
    • platelet type antibody
    see all

Anti-PFKP antibody images

  • Anti-PFKP antibody (ab89003) at 1 µg/ml + Human pancreas tissue lysate at 50 µg

    Predicted band size : 86 kDa
  • Anti-PFKP antibody (ab89003) at 1 µg/ml + Hela nuclear extracts at 50 µg

    Predicted band size : 86 kDa
  • All lanes : Anti-PFKP antibody (ab89003) at 1 µg/ml

    Lane 1 : PFKP-transfected 293T cell lysate
    Lane 2 : non transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 86 kDa
  • ab89003 at 10 µg/ml staining PFKP in Hela cells by immunofluorescence.

References for Anti-PFKP antibody (ab89003)

ab89003 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Heads)
Gel Running Conditions Non-reduced Denaturing
Loading amount 50 µg
Specification Heads
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ernesto Manzo

Verified customer

Submitted Apr 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"