Anti-PFKP antibody [OTI1D6] (ab119796)
Key features and details
- Mouse monoclonal [OTI1D6] to PFKP
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-PFKP antibody [OTI1D6]
See all PFKP primary antibodies -
Description
Mouse monoclonal [OTI1D6] to PFKP -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Recombinant full length Human PFKP produced in HEK293T cells (NP_002618).
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Positive control
- HEK293T cell lysate transfected with pCMV6-ENTRY PFKP; SVT2, A549, Jurkat, PC12 and MCF7 cell extracts; Human colon adenocarcinoma, kidney carcinoma, lung carcinoma, ovary adenocarcinoma, endometrium, endometrium adenocarcinoma, bladder carcinoma and tonsil tissues; COS7 cells transiently transfected with pCMV6-ENTRY PFKP; Jurkat cells.
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General notes
The clone number has been updated from 1D6 to OTI1D6, both clone numbers name the same clone.
Dilute in PBS (pH 7.3) before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI1D6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 86 kDa.
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IHC-P |
1/150.
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ICC/IF |
1/100.
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 86 kDa. |
IHC-P
1/150. |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. -
Sequence similarities
Belongs to the phosphofructokinase family. Two domains subfamily. - Information by UniProt
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Database links
- Entrez Gene: 5214 Human
- Entrez Gene: 56421 Mouse
- Entrez Gene: 60416 Rat
- Omim: 171840 Human
- SwissProt: Q01813 Human
- SwissProt: Q9WUA3 Mouse
- SwissProt: P47860 Rat
- Unigene: 26010 Human
see all -
Alternative names
- 1200015H23Rik antibody
- 6 phosphofructokinase type C antibody
- 6 phosphofructokinase, platelet type antibody
see all
Images
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All lanes : Anti-PFKP antibody [OTI1D6] (ab119796) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PFKP knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDaLanes 1-3: Merged signal (red and green). Green - ab119796 observed at 86 kDa. Red - loading control ab181602 observed at 36 kDa.
ab119796 Anti-PFKP antibody [OTI1D6] was shown to specifically react with PFKP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265136 (knockout cell lysate ab257580) was used. Wild-type and PFKP knockout samples were subjected to SDS-PAGE. ab119796 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Colon tissue by Immunohistochemistry.
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All lanes : Anti-PFKP antibody [OTI1D6] (ab119796) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HeLa cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 cell extract
Lane 5 : COS7 cell extract
Lane 6 : Jurkat cell extract
Lane 7 : MDCK cell extract
Lane 8 : PC12 cell extract
Lane 9 : MCF7 cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 86 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200673) -
All lanes : Anti-PFKP antibody [OTI1D6] (ab119796) at 1/500 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PFKP cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 86 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200673) -
ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Kidney carcinoma tissue by Immunohistochemistry.
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ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Lung carcinoma tissue by Immunohistochemistry.
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ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Ovary adenocarcinoma tissue by Immunohistochemistry.
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ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Endometrium tissue by Immunohistochemistry.
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ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Endometrium adenocarcinoma tissue by Immunohistochemistry.
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ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Bladder carcinoma tissue by Immunohistochemistry.
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ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
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ab119796 at 1/100 dilution staining PFKP in COS7 cells transiently transfected with pCMV6-ENTRY PFKP by Immunofluorescence.
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ab119796 at 1/100 dilution staining PFKP in Jurkat cells by Flow Cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab119796 has been referenced in 13 publications.
- Li Z et al. The PCK2-glycolysis axis assists three-dimensional-stiffness maintaining stem cell osteogenesis. Bioact Mater 18:492-506 (2022). PubMed: 35415308
- Peng J et al. Docetaxel suppressed cell proliferation through Smad3/HIF-1α-mediated glycolysis in prostate cancer cells. Cell Commun Signal 20:194 (2022). PubMed: 36536346
- Kalezic A et al. Tissue-Specific Warburg Effect in Breast Cancer and Cancer-Associated Adipose Tissue-Relationship between AMPK and Glycolysis. Cancers (Basel) 13:N/A (2021). PubMed: 34073074
- Wang F et al. Platelet isoform of phosphofructokinase promotes aerobic glycolysis and the progression of non-small cell lung cancer. Mol Med Rep 23:N/A (2021). PubMed: 33236133
- Wang C et al. Autophagy activated by silibinin contributes to glioma cell death via induction of oxidative stress-mediated BNIP3-dependent nuclear translocation of AIF. Cell Death Dis 11:630 (2020). PubMed: 32801360