Overview

  • Product nameAnti-PGAM1 antibody
    See all PGAM1 primary antibodies
  • Description
    Goat polyclonal to PGAM1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Monkey
  • Immunogen

    Synthetic peptide: KAMEAVAAQGKAKK, corresponding to C terminal amino acids 241-254 of Human PGAM1.

  • Positive control
    • Human liver and brain lysates.
  • General notesGenBank Accession Number – NP_002620.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 28 kDa.Can be blocked with Human PGAM1 peptide (ab22868).
IHC-P Use at an assay dependent concentration. PubMed: 21622897

Target

Anti-PGAM1 antibody images

  • Anti-PGAM1 antibody (ab2220) at 0.01 µg/ml + Human liver lysate at 35 µg
    developed using the ECL technique

    Primary incubated for 1 hour

  • ab2220 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab2220 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ab2220 staining PGAM1 in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    N, normal colonic mucosa; TII, Stage II; and TIV, Stage IV. Positive staining is indicated by red arrows.

References for Anti-PGAM1 antibody (ab2220)

This product has been referenced in:
  • Okuda J  et al. Persistent overexpression of phosphoglycerate mutase, a glycolytic enzyme, modifies energy metabolism and reduces stress resistance of heart in mice. PLoS One 8:e72173 (2013). WB ; Mouse . Read more (PubMed: 23951293) »
  • Hallows WC  et al. Regulation of Glycolytic Enzyme Phosphoglycerate Mutase-1 by Sirt1 Protein-mediated Deacetylation. J Biol Chem 287:3850-8 (2012). WB ; Human . Read more (PubMed: 22157007) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (BRAIN)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 20 µg
Specification BRAIN
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 16 2015

Thank you for your enquiry. I have blasted the immunizing peptide sequence and found that 13 out of 14 amino acids were identical to the sequence of PGAM2. Therefore, it is likely that this product might cross react with PGAM2. However, since we hav...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"