Anti-PGAM1 antibody (ab96622)
Key features and details
- Rabbit polyclonal to PGAM1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PGAM1 antibody
See all PGAM1 primary antibodies -
Description
Rabbit polyclonal to PGAM1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Zebrafish -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 16-209 of Human PGAM1 (AAH00455).
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Positive control
- 293T, A431, Jurkat and Raji cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified by antigen affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 29 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 29 kDa. |
Target
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Function
Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. -
Tissue specificity
In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. -
Sequence similarities
Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily. - Information by UniProt
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Database links
- Entrez Gene: 5223 Human
- Entrez Gene: 18648 Mouse
- Entrez Gene: 24642 Rat
- Omim: 172250 Human
- SwissProt: P18669 Human
- SwissProt: Q9DBJ1 Mouse
- SwissProt: P25113 Rat
- Unigene: 592599 Human
see all -
Alternative names
- BPG dependent PGAM 1 antibody
- BPG dependent PGAM1 antibody
- BPG-dependent PGAM 1 antibody
see all
Images
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All lanes : Anti-PGAM1 antibody (ab96622) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa -
All lanes : Anti-PGAM1 antibody (ab96622) at 1/1000 dilution
Lane 1 :Recombinant Human PGAM1 protein (ab100885) at 0.1 µg
Lane 2 :Recombinant Human PGAM1 protein (ab100885) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Exposure time: 1 minute
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab96622 has been referenced in 3 publications.
- Pirovich DB et al. Schistosoma mansoni phosphoglycerate mutase: a glycolytic ectoenzyme with thrombolytic potential. Parasite 29:41 (2022). PubMed: 36083036
- Park MK et al. NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. Cell Metab 33:2380-2397.e9 (2021). PubMed: 34879239
- Jiang W et al. Proteomic analysis of protein expression profiles during hyperthermia-induced apoptosis in Tca8113 cells. Oncol Lett 6:135-143 (2013). PubMed: 23946791