Recombinant Anti-PGAM2 antibody [EPR15193(B)] (ab183027)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15193(B)] to PGAM2
- Suitable for: Flow Cyt (Intra), IP, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PGAM2 antibody [EPR15193(B)]
See all PGAM2 primary antibodies -
Description
Rabbit monoclonal [EPR15193(B)] to PGAM2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human skeletal muscle lysate; Human fetal heart lysate; Human skeletal muscle tissue; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15193(B) -
Isotype
IgG
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP |
1/30 - 1/50.
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IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/10000 - 1/50000. Predicted molecular weight: 29 kDa.
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Notes |
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Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/30 - 1/50. |
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/10000 - 1/50000. Predicted molecular weight: 29 kDa. |
Target
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Function
Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. -
Tissue specificity
In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. -
Involvement in disease
Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10) [MIM:261670]. A metabolic disorder characterized by myoglobinuria, increased serum creatine kinase levels, decreased phosphoglycerate mutase activity, myalgia, muscle pain, muscle cramps and excercise intolerance. -
Sequence similarities
Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily. - Information by UniProt
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Database links
- Entrez Gene: 5224 Human
- Entrez Gene: 56012 Mouse
- Entrez Gene: 24959 Rat
- Omim: 612931 Human
- SwissProt: P15259 Human
- SwissProt: O70250 Mouse
- SwissProt: P16290 Rat
- Unigene: 632642 Human
see all -
Alternative names
- BPG dependent PGAM 2 antibody
- BPG-dependent PGAM 2 antibody
- GSD10 antibody
see all
Images
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All lanes : Anti-PGAM2 antibody [EPR15193(B)] (ab183027) at 1/20000 dilution
Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 29 kDa -
Immunohistochemistry analysis of paraffin embedded Human skeletal muscle tissue, labeling PGAM2 with ab183027 at 1/500 dilution. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunopreciptation analysis of Human skletal muscle lysate, labeling PGAM2 with ab183027 at 1/50 dilution (lane 1) and negative control (lane 2) Anti-Rabbit IgG (HRP) secondary antibody used at 1/1500 dilution (lane 1) and blocking buffer was 5% NFDM/TBST.
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Intracellular Flow Cytometry analysis of Jurkat cells fixed with 2% paraformaldehyde, labeling PGAM2 with ab183027 at 1/20 dilution. Goatanti Rabbit IgG secondary antibody used at 1/150 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab183027 has not yet been referenced specifically in any publications.