Overview

  • Product name
  • Description
    Rabbit polyclonal to PGAM5
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
  • Immunogen

    antigen sequence: NWDRREPLSL INVRKRNVES GEEELASKLD HYKAKATRHI FLIRHSQYHV DGSLEKDRTL TPLGREQAEL TGLRLASLGL KFNKIV, corresponding to amino acids 61-146 of Human PGAM5.

  • Positive control
    • Human uterus tissue; RT-4 and U-251 MG cell lysates; Human liver and tonsil lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/500 - 1/1000. Predicted molecular weight: 32 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression.
  • Sequence similarities
    Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
  • Domain
    According to PubMed:18387606, may contain a non-cleaved N-terminal mitochondrial targeting sequence that targets PGAM5 to the cytosolic side of the outer mitochondrial membrane, instead of a N-terminal transmembrane.
  • Cellular localization
    Mitochondrion. Mitochondrion outer membrane. Membrane. Isoform 2 overexpression results in the formation of disconnected punctuate mitochondria distributed throughout the cytoplasm. Isoform 1 overexpression results in the clustering of mitochondria around the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bcl-XL-binding protein v68 antibody
    • BXLBv68 antibody
    • MGC5352 antibody
    • mitochondrial antibody
    • PGAM5 antibody
    • PGAM5_HUMAN antibody
    • Phosphoglycerate mutase family member 5 antibody
    • Serine/threonine protein phosphatase PGAM5 mitochondrial antibody
    • Serine/threonine-protein phosphatase PGAM5 antibody
    see all

Images

  • All lanes : Anti-PGAM5 antibody (ab126534) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate


    Predicted band size : 32 kDa
  • Immunofluorescent staining of Human cell line MCF-7 shows positivity in mitochondria. Recommended concentration of ab126534 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab126534, at 1/200 dilution, staining PGAM5 in paraffin-embedded Human uterus tissue by Immunohistochemistry.

References

This product has been referenced in:
  • Moriwaki K  et al. The Mitochondrial Phosphatase PGAM5 Is Dispensable for Necroptosis but Promotes Inflammasome Activation in Macrophages. J Immunol 196:407-15 (2016). Mouse . Read more (PubMed: 26582950) »
  • Lohr K  et al. Reduced mitochondrial mass and function add to age-related susceptibility toward diet-induced fatty liver in C57BL/6J mice. Physiol Rep 4:N/A (2016). WB . Read more (PubMed: 27694529) »

See all 6 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (thymus and spleen)
Loading amount
30 µg
Specification
thymus and spleen
Gel Running Conditions
Reduced Denaturing (10 Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Apr 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Tissue lysate - whole (whole embryo)
Loading amount
15 µg
Specification
whole embryo
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 27 2012

ab67992 : DISCOUNT CODE: ABTRIAL-000

ab126534 : DISCOUNT CODE: ABTRIAL-000

Ablaufdatum: 05. März 2013

Es freut mich zu hören, dass Sie unser Angebot unsere Antikörper ab67992 und ab126534 in Xenopus zu teste...

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Gracias por contactarnos.



El anticuerpo ab126534 solo se ha testado en nuestro laboratorio en muestras humanas, lo cual no quiere decir que no vaya a reconocer la proteína en otras especies, pero al no tener datos para probar...

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Thank you for contacting Abcam regarding ab126534.


The Anti-PGAM5 antibody can recognize both the PGAM5-001 (ENSP00000321503) and PGAM5-005 (ENSP00000438465) isoforms, since the antigen sequence has 100% sequence identity to these two tra...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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