Overview

  • Product nameAnti-PGBD3 antibody
  • Description
    Rabbit polyclonal to PGBD3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within Synthetic peptide: SDSDDPSYAP KDDSPDEVPS TFTVQQPPPS RRRKMTKILC KWKKADLTVQ, corresponding to N terminal amino acids 73-122 of Human PGBD3

  • Positive control
    • Jurkat cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • RelevanceThe piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes, including human, that appear to have been derived from the piggyBac transposons. PGBD3 belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families. PGBD3 overlaps with the ERCC6 gene on chromosome 10, and pseudogenes of this locus have been found on chromosomes 4, 5 and 12.
  • Database links
  • Alternative names
    • FLJ90201 antibody
    • Hypothetical protein LOC267004 antibody
    • PGBD 3 antibody
    • PiggyBac transposable element derived 3 antibody
    • PiggyBac transposable element derived protein 3 antibody
    see all

Anti-PGBD3 antibody images

  • Anti-PGBD3 antibody (ab66903) at 2.5 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.

References for Anti-PGBD3 antibody (ab66903)

ab66903 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"